Andersen M L, Ribeiro D A, Bergamaschi C T, Alvarenga T A, Silva A, Zager A, Campos R R, Tufik S
Department of Psychobiology, Universidade Federal de São Paulo (UNIFESP), Brazil.
Prog Neuropsychopharmacol Biol Psychiatry. 2009 Apr 30;33(3):562-7. doi: 10.1016/j.pnpbp.2009.02.014. Epub 2009 Feb 28.
The aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) and long-term (21 days) intervals, as well as their respective recovery periods in peripheral blood, brain, liver and heart tissue by the single cell gel (comet) assay. Rats were paradoxically deprived of sleep (PSD) by the platform technique for 24 or 96 h, or chronically sleep-restricted (SR) for 21 days. We also sought to verify the time course of their recovery after 24 h of rebound sleep. The results showed DNA damage in blood cells of rats submitted to PSD for 96 h. Brain tissue showed extensive genotoxic damage in PSD rats (both 24 and 96 h), though the effect was more pronounced in the 96 h group. Rats allowed to recover from the PSD-96 h and SR-21 days treatments showed DNA damage as compared to negative controls. Liver and heart did not display any genotoxicity activity. Corticosterone concentrations were increased after PSD (24 and 96 h) relative to control rats, whereas these levels were unaffected in the SR group. Collectively, these findings reveal that sleep loss was able to induce genetic damage in blood and brain cells, especially following acute exposure. Since DNA damage is an important step in events leading to genomic instability, this study represents a relevant contribution to the understanding of the potential health risks associated with sleep deprivation.
本研究的目的是通过单细胞凝胶（彗星）试验，评估实验性睡眠剥夺在短期（24小时和96小时）和长期（21天）对雄性大鼠诱导的遗传损伤，以及它们在外周血、脑、肝和心脏组织中的各自恢复期。通过平台技术使大鼠反常睡眠剥夺（PSD）24或96小时，或慢性睡眠限制（SR）21天。我们还试图验证其在24小时反弹睡眠后的恢复时间进程。结果显示，接受96小时PSD的大鼠血细胞中有DNA损伤。脑组织在PSD大鼠（24小时和96小时）中显示出广泛的遗传毒性损伤，尽管在96小时组中这种效应更明显。与阴性对照组相比，从PSD-96小时和SR-21天处理中恢复的大鼠显示出DNA损伤。肝和心脏未显示任何遗传毒性活性。与对照大鼠相比，PSD（24小时和96小时）后皮质酮浓度升高，而SR组中这些水平未受影响。总的来说，这些发现表明睡眠剥夺能够在血液和脑细胞中诱导遗传损伤，尤其是在急性暴露后。由于DNA损伤是导致基因组不稳定事件中的重要一步，本研究为理解与睡眠剥夺相关的潜在健康风险做出了相关贡献。
