Department of Psychobiology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Basic Clin Pharmacol Toxicol. 2010 Jul;107(1):598-602. doi: 10.1111/j.1742-7843.2010.00540.x. Epub 2010 Feb 9.
The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. The hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. The same lack of effect was noted in kidney and heart cells of all strains evaluated. In conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose.
本研究的目的是通过单细胞凝胶（彗星）试验检测三种不同雄性小鼠在周围血液、心脏、肾脏和肝脏组织中由反常性睡眠剥夺（PSD）引起的遗传损伤。将瑞士、C57BL/6j 和无毛（HRS/j）小鼠通过多平台技术进行 72 小时 PSD，评估 DNA 损伤。与阴性对照相比，瑞士小鼠的血细胞中发现 DNA 损伤有统计学意义的差异。相比之下，C57BL/6j 或无毛小鼠的基因未发生统计学差异。在肝脏方面，瑞士小鼠中发现了广泛的遗传毒性作用。无毛和 C57BL/6j 小鼠在该器官中未显示出任何遗传毒性迹象。在所评估的所有菌株的肾脏和心脏细胞中也观察到相同的无效应。总之，我们的结果表明，睡眠剥夺仅在瑞士小鼠的血液和肝脏细胞中以 DNA 断裂的形式造成遗传损伤。在研究睡眠剥夺对小鼠遗传装置的有害作用时，应该考虑这种方法，因为瑞士品系更适合于这一目的。
