Department of Psychobiology, Universidade Federal de São Paulo, Vila Clementino, SP, São Paulo, Brazil.
Horm Behav. 2010 Feb;57(2):216-21. doi: 10.1016/j.yhbeh.2009.11.004. Epub 2009 Dec 1.
The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. The results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. In ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. In conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones.
本研究旨在评估实验性反常性睡眠剥夺（PSD）对动情周期和卵巢切除雌性大鼠整体 DNA 损伤的影响，以探讨 DNA 损伤过程中可能存在的激素参与。将处于动情周期不同阶段（发情前期、发情期和发情后期）的完整大鼠或卵巢切除的 Wistar 雌性大鼠用单平台技术进行 PSD 96 小时，或在自家笼中作为对照维持等效时间。在此期间，收集外周血和组织（脑、肝和心脏），使用单细胞凝胶（彗星）试验评估遗传损伤。结果表明，PSD 导致暴露于 PSD 96 小时的大鼠脑细胞发生广泛的遗传毒性作用，表现为 DNA 迁移率增加，与阴性对照组相比。这在动情周期的所有阶段都观察到，没有明显的区别。在卵巢切除大鼠中，PSD 也导致脑细胞的 DNA 损伤。对所有分析的组，在外周血、肝脏或心脏中均未检测到统计学上显著差异。总之，我们的数据与以下观点一致，即睡眠剥夺引起的脑细胞 DNA 断裂形式的遗传损伤超过了与内源性雌性性激素相关的影响。
