脂蛋白脂肪酶基因中PvuII多态性与马其顿人群冠状动脉疾病的关联。

Association of PvuII polymorphism in the lipoprotein lipase gene with the coronary artery disease in Macedonian population.

作者信息

Georgiev Antonio, Panov S, Sadikario S

机构信息

Heart Diseases Institute, University Clinical Centre, Medical Faculty, Ss. Cyril and Methodius University, Skopje, R. Macedonia.

出版信息

Prilozi. 2008 Dec;29(2):213-25.

PMID:19259048

Abstract

UNLABELLED

In the etiology of coronary artery disease there are many factors involved as a result of the complex interaction between genetic predisposition and environmental influences. The lipoprotein lipase (LPL) plays a very important role in lipid metabolism. It hydrolyzes the triglycerides in hylomicrones and very low density lipoproteins - VLDL. PvuII polymorphism in the LPL gene is a frequent variant and it increases triglyceride levels and the risk of the appearance of coronary arterial disease.

AIM

The aim of this work is to show LPL-PvuII polymorphism as an independent risk factor and also as a predictor of coronary arterial disease in the Macedonian population.

MATERIAL AND METHODS

The study included 109 randomized patients with coronary artery disease (CAD) (83 males, 26 females), treated at the Cardiology Clinic. The stenosis of coronary arteries greater than 70% of the artery lumen was angiographically documented in the CAD group. The control group consisted of 32 patients (25 males, 7 females) with documented normal coronarographic findings. The patients' age ranged from 50 to 59; the mean age in the CAD group was 59.4 and the mean age in the control group was 57.9. LPL-PvuII polymorphism in the intron 6 in the CAD and control group was detected by PCR amplification and restriction enzyme digestion.

RESULTS

A statistically significant association between CAD and the control group was found regarding the presence of hyperlipidaemia (p < 0.001), diabetes (p < 0.05) and the use of antilipidaemic drugs (p < 0.049). The presence of LPL-PvuII polymorphism in both investigated groups does not represent a statistically significant risk factor for the appearance of coronary artery disease (p = 0.816). The PvuII + allele frequency of 0.495 and 0,469 was obtained in both the angiographically confirmed CAD and the control groups, respectively. This finding indicates no significant differences between the prevalence of the LPL-PvuII genotypes in both study groups, suggesting a lack of association of LPL-PvuII polymorphism with CAD. However, the homozygous genotype (PvuII +/+) was more prevalent in the CAD group (22.9%) in comparison with the control group (15.6%).

CONCLUSION

In our study LPL-PvuII polymorphism was not identified as an independent risk factor for the appearance of CAD.

摘要

未标注

在冠状动脉疾病的病因中，由于遗传易感性和环境影响之间复杂的相互作用，涉及许多因素。脂蛋白脂肪酶（LPL）在脂质代谢中起着非常重要的作用。它水解乳糜微粒和极低密度脂蛋白（VLDL）中的甘油三酯。LPL基因中的PvuII多态性是一种常见变异，它会增加甘油三酯水平以及冠状动脉疾病出现的风险。

目的

本研究的目的是表明LPL - PvuII多态性是马其顿人群中冠状动脉疾病的独立危险因素及预测指标。

材料与方法

该研究纳入了在心脏病诊所接受治疗的109例随机选取的冠状动脉疾病（CAD）患者（83例男性，26例女性）。CAD组经血管造影记录显示冠状动脉狭窄超过动脉管腔的70%。对照组由32例患者（25例男性，7例女性）组成，冠状动脉造影结果显示正常。患者年龄在50至59岁之间；CAD组的平均年龄为59.4岁，对照组的平均年龄为57.9岁。通过PCR扩增和限制性内切酶消化检测CAD组和对照组中第6内含子的LPL - PvuII多态性。

结果

在高脂血症（p < 0.001）、糖尿病（p < 0.05）和使用抗血脂药物（p < 0.049）方面，CAD组与对照组之间存在统计学上的显著关联。在两个研究组中，LPL - PvuII多态性的存在并不代表冠状动脉疾病出现的统计学显著危险因素（p = 0.816）。在血管造影确诊的CAD组和对照组中，PvuII +等位基因频率分别为0.495和0.469。这一发现表明两个研究组中LPL - PvuII基因型的患病率无显著差异，提示LPL - PvuII多态性与CAD缺乏关联。然而，纯合基因型（PvuII +/+）在CAD组中（22.9%）比对照组（15.6%）更常见。