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松材线虫携带的荧光假单胞菌GcM5-1A产生的两种环二肽及其对日本黑松悬浮细胞和幼苗的体外毒性。

Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro.

作者信息

Guo Qunqun, Guo Daosen, Zhao Boguang, Xu Jie, Li Ronggui

机构信息

Department of Biology, Qingdao University, Qingdao 266071, P. R. China Department of Forest Entomology and Pathology, Nanjing Forest University, Nanjing 210037, P. R. China.

出版信息

J Nematol. 2007 Sep;39(3):243-7.

PMID:19259494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2586505/
Abstract

Pseudomonas fluorescens GcM5-1A, isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, was cultured in Luria Broth medium (LB). The clarified culture was extracted with ethyl acetate, and two dipeptides were purified from the extract. The chemical structures of 1 and 2 were identified as cyclo(-Pro-Val-)and cyclo(-Pro-Tyr-), respectively, by MS, (1)H NMR, (13)C NMR,(1)H-(1)H COSY, 1H -(13)C COSY spectra. Bioassay results showed that the two compounds were toxic to both suspension cells and seedlings of Pinus thunbergii, which may offer some clues to research the mechanism of pine wilt disease caused by PWN.

摘要

从松材线虫（Bursaphelenchus xylophilus）中分离出的荧光假单胞菌GcM5-1A，在Luria肉汤培养基（LB）中培养。将澄清的培养物用乙酸乙酯萃取，从萃取物中纯化出两种二肽。通过质谱、（1）H核磁共振、（13）C核磁共振、（1）H-（1）H化学位移相关谱、1H-（13）C化学位移相关谱确定化合物1和2的化学结构分别为环（-脯氨酸-缬氨酸-）和环（-脯氨酸-酪氨酸-）。生物测定结果表明，这两种化合物对黑松的悬浮细胞和幼苗均有毒性，这可能为研究松材线虫引起的松材萎蔫病的发病机制提供一些线索。

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Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro.松材线虫携带的荧光假单胞菌GcM5-1A产生的两种环二肽及其对日本黑松悬浮细胞和幼苗的体外毒性。
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本文引用的文献

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Pathogenesis in Pine Wilt Caused by Pinewood Nematode, Bursaphelenchus xylophilus.松材线虫（Bursaphelenchus xylophilus）引起的松材枯萎病发病机制
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