Hoch J R, Stevens R P, Keller M P, Silver D
Department of Surgery, University of Missouri Health Sciences Center, Columbia 65212.
Surgery. 1991 Oct;110(4):656-62; discussion 662-3.
The ability of mannitol and superoxide dismutase (SOD) to improve the recovery of peripheral nerve and skeletal muscle function and to influence metabolism during reperfusion after 4 hours of complete ischemia was investigated in an autoperfused canine hind-limb model. Study groups included control subjects (n = 7), subjects given 5000 units/kg of SOD intra-arterial bolus immediately before reperfusion and 10,000 units/kg infusion during first hour of reperfusion (n = 7), and subjects given 150 mg/kg isosmolar mannitol intra-arterial bolus before reperfusion and 1 gm/kg intravenous infusion during the first hour of reperfusion. Function was evaluated by determining isometric twitch and tetanic contractile force of paw dorsiflexion by stimulating the peroneal nerve or the anterior tibial muscle. Metabolic responses (oxygen consumption and lactate clearance) and blood flow were not influenced by either treatment protocol. However, mannitol significantly reduced muscle damage and significantly improved neuromuscular contractile function compared to control and SOD treatment regimens.
在一个自体灌注犬后肢模型中，研究了甘露醇和超氧化物歧化酶（SOD）在完全缺血4小时后再灌注期间改善周围神经和骨骼肌功能恢复以及影响代谢的能力。研究组包括对照组（n = 7）、在再灌注前立即给予5000单位/千克SOD动脉内推注并在再灌注第一小时给予10000单位/千克输注的组（n = 7），以及在再灌注前给予150毫克/千克等渗甘露醇动脉内推注并在再灌注第一小时给予1克/千克静脉输注的组。通过刺激腓总神经或胫前肌来测定爪背屈的等长收缩和强直收缩力，以此评估功能。两种治疗方案均未影响代谢反应（耗氧量和乳酸清除率）及血流量。然而，与对照组和SOD治疗方案相比，甘露醇显著减少了肌肉损伤并显著改善了神经肌肉收缩功能。
