Heinemeyer E-A, Luden K
Niedersächsisches Landesgesundheitsamt, Aussenstelle Aurich, Aurich, BRD.
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2009 Mar;52(3):345-51. doi: 10.1007/s00103-009-0797-5.
Pseudomonas aeruginosa (P. aeruginosa) is a relevant pathogen to bathers and, therefore, water from natural swimming pools is analyzed for its presence. P. aeruginosa can be detected regularly in surface waters. Currently the methods described in DIN EN 12780 are recommended for the quantitative analysis of water from natural swimming pools, although this standard was developed for bottled water. The present study examines whether the recommendation is satisfactory. In 93 water samples from five natural swimming pools (Naturschwimmteiche), 3226 presumptive P. aeruginosa colonies were detected. Of the 3226 colonies, 101 were directly confirmed due to their formation of pyocyanin. From the remaining 3125 colonies, 349 representative samples were isolated and examined further. Using the criteria of DIN EN 12780 fluorescense and ammonia formation from acetamide, another 45 colonies were considered to be P. aeruginosa. 71% (32) of these 45 non-pyocyanin-forming strains could not be confirmed by the APINE system. None of the 32 strains grew at 42 degrees C, which is a characteristic feature of P. aeruginosa as mentioned in a note of DIN EN 12780. In 20 of the original samples, P. aeruginosa numbers exceeded the recommended limit value of 10/100 ml. Eleven cases (55 %) were caused by P. aeruginosa, which were not confirmed taxonomically. Due to insufficient selectivity, the method according to DIN EN 12780 is not applicable for the testing of surface waters and water from natural swimming pools. The high number of false positive results for P. aeruginosa questions the standard even further. It is suggested that for surface water samples growth of P. aeruginosa at 42 degrees C has to be checked as an additional confirmation characteristics of P. aeruginosa until a reliable standard is published.
铜绿假单胞菌是与游泳者相关的病原体，因此需要对天然游泳池的水进行该菌存在情况的分析。在地表水中可经常检测到铜绿假单胞菌。目前推荐采用DIN EN 12780中描述的方法对天然游泳池的水进行定量分析，尽管该标准是针对瓶装水制定的。本研究考察该推荐方法是否令人满意。在取自5个天然游泳池的93份水样中，检测到3226个疑似铜绿假单胞菌菌落。在这3226个菌落中，有101个因其产生绿脓菌素而被直接确认为铜绿假单胞菌。从其余3125个菌落中，分离出349个代表性样本并进一步检测。根据DIN EN 12780中荧光和由乙酰胺生成氨的标准，另外45个菌落被认定为铜绿假单胞菌。这45个不产生绿脓菌素的菌株中有71%（32个）不能通过APINE系统得到确认。这32个菌株均不能在42℃生长，而这是DIN EN 12780注释中提到的铜绿假单胞菌的一个特征。在20份原始样本中，铜绿假单胞菌数量超过了推荐的限值10/100 ml。有11例（55%）是由未通过分类学确认的铜绿假单胞菌引起的。由于选择性不足，DIN EN 12780方法不适用于地表水和天然游泳池水的检测。铜绿假单胞菌出现大量假阳性结果，这进一步对该标准提出质疑。建议对于地表水样本，在可靠标准发布之前，必须检查铜绿假单胞菌在42℃的生长情况，作为铜绿假单胞菌的一项额外确认特征。
