Miyamoto Ayako, Taniguchi Fuminori, Tagashira Yukiko, Watanabe Ayako, Harada Tasuku, Terakawa Naoki
Department of Obstetrics and Gynecology, Tottori University School of Medicine, Nishimachi, Yonago, Japan.
Am J Reprod Immunol. 2009 Apr;61(4):277-85. doi: 10.1111/j.1600-0897.2009.00691.x.
We previously reported that lipopolysaccharide (LPS)-promoted endometriotic stromal cell (ESC) proliferation by inducing TNFalpha production. The aim of this study was to investigate the efficacy of TNFalpha gene silencing on LPS-treated ESCs.
Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS-induced gene expression profiling after transfection of TNFalpha siRNA into ESCs was performed. Down-regulated genes by TNFalpha silencing were examined using real-time RT-PCR. Effect of TNFalpha silencing was examined using ELISA and BrdU incorporation, respectively.
In PCR array, TNFalpha silencing in ESCs repressed LPS-induced expression of cIAP2 and IL-8, NFkappaB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL-8 expression in ESCs were higher compared with those in EMSCs. TNFalpha silencing attenuated the LPS-induced ESC proliferation.
Tumor necrosis factor alpha may be involved in cell proliferation of endometriotic tissues.
我们之前报道过，脂多糖（LPS）通过诱导肿瘤坏死因子α（TNFα）的产生促进子宫内膜异位症间质细胞（ESC）增殖。本研究的目的是探讨TNFα基因沉默对LPS处理的ESC的影响。
分别从卵巢巧克力囊肿和子宫肌瘤中获取子宫内膜异位症间质细胞（ESC）和子宫内膜间质细胞（EMSC）。利用PCR阵列技术，对转染TNFα siRNA后的ESC进行LPS诱导的基因表达谱分析。采用实时RT-PCR检测TNFα沉默后下调的基因。分别用ELISA和BrdU掺入法检测TNFα沉默的效果。
在PCR阵列中，ESC中的TNFα沉默抑制了LPS诱导的细胞凋亡抑制蛋白2（cIAP2）和白细胞介素8（IL-8）的表达，这两种基因是核因子κB（NFκB）信号通路的反应基因。添加LPS后，ESC中cIAP2和IL-8的表达水平高于EMSC。TNFα沉默减弱了LPS诱导的ESC增殖。
肿瘤坏死因子α可能参与子宫内膜异位组织的细胞增殖。
