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FliJ 护送同源物 InvI 和 YscO 对毒力 III 型分泌伴侣蛋白的选择性结合。

Selective binding of virulence type III export chaperones by FliJ escort orthologues InvI and YscO.

作者信息

Evans Lewis D B, Hughes Colin

机构信息

Department of Pathology, Cambridge University, Cambridge, UK.

出版信息

FEMS Microbiol Lett. 2009 Apr;293(2):292-7. doi: 10.1111/j.1574-6968.2009.01535.x. Epub 2009 Mar 2.

DOI:10.1111/j.1574-6968.2009.01535.x
PMID:19260965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3500872/
Abstract

Bacteria secrete flagella subunits and deliver virulence effectors via type III export systems. During flagellar filament assembly, a chaperone escort mechanism has been proposed to enhance the export of early, minor flagellar filament components by selectively binding and cycling their chaperones. Here we identify virulence orthologues of the flagellar chaperone escort FliJ and show that the orthologues Salmonella InvI and Yersinia YscO are, like FliJ, essential for their type III export pathway and similarly, do not bind export substrates. Like FliJ, they recognize a subset of export chaperones, in particular those of the host membrane translocon components required for subsequent effector delivery.

摘要

细菌通过III型分泌系统分泌鞭毛亚基并传递毒力效应蛋白。在鞭毛丝组装过程中，有人提出一种伴侣护送机制，通过选择性结合和循环伴侣蛋白来增强早期少量鞭毛丝成分的输出。在这里，我们鉴定了鞭毛伴侣护送蛋白FliJ的毒力同源物，并表明同源物沙门氏菌InvI和耶尔森氏菌YscO与FliJ一样，对其III型分泌途径至关重要，同样，它们不结合分泌底物。与FliJ一样，它们识别一部分分泌伴侣蛋白，特别是那些后续效应蛋白传递所需的宿主膜转运体成分的伴侣蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/2fa746fc0fb8/fml0293-0292-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/aa44c0fc1a2e/fml0293-0292-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/6fcf08f973ed/fml0293-0292-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/2fa746fc0fb8/fml0293-0292-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/aa44c0fc1a2e/fml0293-0292-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/6fcf08f973ed/fml0293-0292-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9364/3500872/2fa746fc0fb8/fml0293-0292-f3.jpg

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