Vijayaraghavan R, Sugendran K, Pant S C, Husain K, Malhotra R C
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Toxicology. 1991;69(1):35-42. doi: 10.1016/0300-483x(91)90151-p.
The influence of dermal application of sulphur mustard (SM) on hepatic lipid peroxidation and the protective effect of flavonoids in SM toxicity was investigated. SM applied on the skin of mice (0.25 or 0.5 LD50) depleted glutathione (GSH) in blood and liver. Malondialdehyde (MDA) levels in the liver showed an increase indicating lipid peroxidation. Administration of vitamin E or two flavonoids, gossypin (GN) and hydroxyethyl rutosides (HR) after dermal application of SM did not alter depletion of GSH but did reduce the MDA level significantly. Survival time of mice with 1 LD50 SM applied dermally was increased by GN and HR to a greater extent than by vitamin E or sodium thiosulphate probably due to one or more of the analgesic, anti-inflammatory, antihepatotoxic, antihistaminic, mast cell stabilization, lipid peroxidation inhibitory and free radical scavenging actions of the flavonoids. The present study indicates that dermally applied SM can induce lipid peroxidation and GSH depletion, and flavonoids may be beneficial in reducing the toxicity.
研究了皮肤涂抹芥子气（SM）对肝脏脂质过氧化的影响以及黄酮类化合物对SM毒性的保护作用。涂抹于小鼠皮肤的SM（0.25或0.5 LD50）使血液和肝脏中的谷胱甘肽（GSH）耗竭。肝脏中的丙二醛（MDA）水平升高，表明发生了脂质过氧化。皮肤涂抹SM后给予维生素E或两种黄酮类化合物，即棉皮素（GN）和羟乙基芦丁（HR），并未改变GSH的耗竭，但显著降低了MDA水平。经皮涂抹1 LD50 SM的小鼠的存活时间，GN和HR的延长程度大于维生素E或硫代硫酸钠，这可能是由于黄酮类化合物具有一种或多种镇痛、抗炎、抗肝毒性、抗组胺、肥大细胞稳定、脂质过氧化抑制和自由基清除作用。本研究表明，经皮涂抹SM可诱导脂质过氧化和GSH耗竭，而黄酮类化合物可能有助于降低毒性。
