Muniandy Sekaran, Qvist Rajes, Yan Gracie Ong Siok, Bee Chook Jack, Chu Yiaw Koon, Rayappan Arokiasamy Vinsent
Department of Molecular Medicine, University of Malaya, Kuala Lumpur, Malaysia.
J Med Invest. 2009 Feb;56(1-2):6-10. doi: 10.2152/jmi.56.6.
Hyperglycemia and insulin resistance are common in many critically ill patients. Hyperglycemia increases the production of reactive oxygen species in cells, stimulates the production of the potent proinflammatory cytokines IL-8 and TNF-alpha, and enhances the expression of haem oxygenase-1, an inducible stress protein. It has been shown that administration of insulin and the semi-essential amino acid glutamine have been beneficial to the septic patient. The aim of our study is to test whether these two molecules, glutamine and insulin used in combination attenuate the proinflammatory responses in endothelial cells which have been triggered by hyperglycaemia. Our results demonstrate that a combination of insulin and glutamine are significantly more effective in reducing the expression of IL-8, TNF-alpha and HO-1 than insulin or glutamine alone.
高血糖和胰岛素抵抗在许多重症患者中很常见。高血糖会增加细胞中活性氧的产生，刺激强效促炎细胞因子IL-8和TNF-α的产生，并增强血红素加氧酶-1（一种诱导性应激蛋白）的表达。研究表明，给予胰岛素和半必需氨基酸谷氨酰胺对脓毒症患者有益。我们研究的目的是测试谷氨酰胺和胰岛素联合使用是否能减弱高血糖引发的内皮细胞促炎反应。我们的结果表明，胰岛素和谷氨酰胺联合使用在降低IL-8、TNF-α和HO-1的表达方面比单独使用胰岛素或谷氨酰胺显著更有效。
