Guermouche B, Soulimane-Mokhtari N A, Bouanane S, Merzouk H, Merzouk S, Narce M
Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou Bekr Belkaid, BP 119, 13000 Tlemcen, Algeria.
Department of Technical Sciences, Faculty of Engineering, University of Abou Bekr Belkaid, 13000 Tlemcen, Algeria.
Biomed Res Int. 2014;2014:368107. doi: 10.1155/2014/368107. Epub 2014 Jun 2.
The aim of this work was to determine the effect of dietary n - 3 PUFA on oxidant/antioxidant status, in vitro very low and low density lipoprotein (VLDL-LDL), and VLDL-LDL-fatty acid composition in macrosomic pups of diabetic mothers. We hypothesized that n - 3 PUFA would improve oxidative stress in macrosomia. Diabetes was induced in female Wistar rats fed with the ISIO diet (control) or with the EPAX diet (enriched in n - 3 PUFAs), by streptozotocin. The macrosomic pups were killed at birth (day 0) and at adulthood (day 90). Lipid parameters and VLDL-LDL-fatty acid composition were investigated. The oxidant/antioxidant status was determined by measuring plasma oxygen radical absorbance capacity (ORAC), hydroperoxides, carbonyl proteins, and VLDL-LDL oxidation. Macrosomic rats of ISIO fed diabetic mothers showed an increase in plasma and VLDL-LDL-triglycerides and VLDL-LDL-cholesterol levels and altered VLDL-LDL-fatty acid composition. Plasma ORAC was low with high hydroperoxide and carbonyl protein levels. The in vitro oxidizability of VLDL-LDL was enhanced in these macrosomic rats. The EPAX diet corrected lipid parameters and improved oxidant/antioxidant status but increased VLDL-LDL susceptibility to oxidation. Macrosomia is associated with lipid abnormalities and oxidative stress. n - 3 PUFA exerts favorable effects on lipid metabolism and on the oxidant/antioxidant status of macrosomic rats. However, there are no evident effects on VLDL-LDL oxidation.
本研究旨在确定膳食n-3多不饱和脂肪酸（PUFA）对糖尿病母亲所生巨大儿的氧化/抗氧化状态、体外极低密度脂蛋白和低密度脂蛋白（VLDL-LDL）以及VLDL-LDL脂肪酸组成的影响。我们假设n-3 PUFA可改善巨大儿的氧化应激。通过链脲佐菌素诱导喂食ISIO饮食（对照）或EPAX饮食（富含n-3 PUFAs）的雌性Wistar大鼠患糖尿病。巨大儿在出生时（第0天）和成年期（第90天）处死。研究脂质参数和VLDL-LDL脂肪酸组成。通过测量血浆氧自由基吸收能力（ORAC）、氢过氧化物、羰基蛋白和VLDL-LDL氧化来确定氧化/抗氧化状态。喂食ISIO饮食的糖尿病母亲所生巨大儿的血浆和VLDL-LDL甘油三酯以及VLDL-LDL胆固醇水平升高，且VLDL-LDL脂肪酸组成改变。血浆ORAC较低，氢过氧化物和羰基蛋白水平较高。这些巨大儿的VLDL-LDL体外氧化能力增强。EPAX饮食纠正了脂质参数并改善了氧化/抗氧化状态，但增加了VLDL-LDL的氧化敏感性。巨大儿与脂质异常和氧化应激有关。n-3 PUFA对巨大儿的脂质代谢和氧化/抗氧化状态具有有利影响。然而，对VLDL-LDL氧化没有明显影响。
