Zhang Yue, Liu Hua, Li Bei, Zhang Jian-Tao, Li Yizhou, Zhang Hongxia
National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China.
Transgenic Res. 2009 Aug;18(4):607-19. doi: 10.1007/s11248-009-9251-6. Epub 2009 Mar 5.
The aim of this research was to generate selectable marker-free transgenic tomato plants with improved tolerance to abiotic stress. An estradiol-induced site-specific DNA excision of a selectable marker gene using the Cre/loxP DNA recombination system was employed to develop transgenic tomato constitutively expressing AtIpk2 beta, an inositol polyphosphate 6-/3-kinase gene from Arabidopsis thaliana. Transgenic tomato plants containing a selectable marker were also produced as controls. The expression of AtIpk2 beta conferred improved resistance to drought, cold and oxidative stress in both sets of transgenic tomato plants. These results demonstrate the feasibility of using this Cre/loxP-based marker elimination strategy to generate marker-free transgenic crops with improved stress tolerance.
本研究的目的是培育对非生物胁迫耐受性增强的无选择标记转基因番茄植株。利用Cre/loxP DNA重组系统，通过雌二醇诱导选择标记基因的位点特异性DNA切除，培育出组成型表达拟南芥肌醇多磷酸6-/3-激酶基因AtIpk2 beta的转基因番茄。还培育了含有选择标记的转基因番茄植株作为对照。AtIpk2 beta的表达使两组转基因番茄植株对干旱、寒冷和氧化胁迫的抗性增强。这些结果证明了使用这种基于Cre/loxP的标记消除策略来培育具有增强胁迫耐受性的无标记转基因作物的可行性。
