Pormsila Worapan, Krähenbühl Stephan, Hauser Peter C
Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Anal Chim Acta. 2009 Mar 23;636(2):224-8. doi: 10.1016/j.aca.2009.02.012. Epub 2009 Feb 14.
The suitability of capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C(4)D) for the direct determination of uric acid in human plasma and urine was investigated. It was found that a careful optimization of the buffer composition and pH was necessary to achieve selective determination in the complex sample matrices. An electrolyte solution consisting of 10mM 2-morpholinoethanesulfonic acid (MES), 10mM histidine and 0.1mM hexadecyltrimethylammonium bromide (CTAB), pH 6.0, was finally found suitable for use as running buffer for both sample matrices. The limit of detection (3 S/N) was determined as 3.3 microM. The linearity of the response was tested for the range between 10 and 500 microM and a correlation coefficient of 0.9996 was obtained. Intra- and inter-day variabilities were <10%. Quantitative analysis of urine and plasma samples showed a good correlation with the routine enzymatic method currently used at the University Hospital of Basel.
研究了采用电容耦合非接触电导检测（C(4)D）的毛细管电泳（CE）直接测定人血浆和尿液中尿酸的适用性。结果发现，为了在复杂的样品基质中实现选择性测定，需要仔细优化缓冲液的组成和pH值。最终发现，由10mM 2-吗啉乙磺酸（MES）、10mM组氨酸和0.1mM十六烷基三甲基溴化铵（CTAB）组成、pH值为6.0的电解质溶液适合用作两种样品基质的运行缓冲液。检测限（3S/N）确定为3.3 microM。在10至500 microM范围内测试了响应的线性，获得的相关系数为0.9996。日内和日间变异均<10%。尿液和血浆样品的定量分析与巴塞尔大学医院目前使用的常规酶法显示出良好的相关性。
