Su Yuhua, Huang Weixiong, Hu Rongzong, Ding Haodong, Hu Kangkang
Department of Chemistry, College of Chemistry and Chemical Engineering, The Key Laboratory of Analytical Sciences of the Ministry of Education, Xiamen University, Xiamen 361005, China.
Biosens Bioelectron. 2009 Apr 15;24(8):2665-70. doi: 10.1016/j.bios.2009.01.037. Epub 2009 Feb 2.
In this work, the anion exchange resin (AER) was modified with a layer of glucose oxidase (GOD) and poly(diallyldimethylammonium chloride) (PDDA), respectively, via layer-by-layer electrostatic self-assembling strategy. The PDDA and GOD modified AER (PDDA/GOD/AER) was then packed into a home-made amperometric cell for flow injection analysis (FIA) of glucose. This design simplified the setup by integrating the enzyme reactor into the amperometric cell. And the AER in the cell behaved bifunctional, it was not only the support of enzymes, but also an anti-interference tool due to its retention effect toward ascorbic acid (AA) and uric acid (UA). A platinum modified porous titanium (Pt/PTi) electrode was utilized in the cell as the working electrode (WE), due to its large effective surface area it could increase the response by 8.3 times as compared with the planar pure platinum electrode. The proposed biosensor was very sensitive (22.4 microA cm(-2) mM(-1)) in glucose quantification, and the linear range was from 1 micromol L(-1) to 2 mmol L(-1) with the detection limit of 0.8 micromol L(-1). The biosensor was used for serum glucose determination, and the result obtained was satisfying. This work may have provided a reference design of the amperometric cell which could be adopted in other enzymatic-FIA biosensors.
在本工作中，通过层层静电自组装策略，分别用一层葡萄糖氧化酶（GOD）和聚二烯丙基二甲基氯化铵（PDDA）对阴离子交换树脂（AER）进行了改性。然后将PDDA和GOD改性的AER（PDDA/GOD/AER）填充到自制的安培池中，用于葡萄糖的流动注射分析（FIA）。这种设计通过将酶反应器集成到安培池中简化了装置。并且池中AER具有双重功能，它不仅是酶的载体，还因其对抗坏血酸（AA）和尿酸（UA）的保留作用而成为一种抗干扰工具。池中使用铂修饰的多孔钛（Pt/PTi）电极作为工作电极（WE），由于其有效表面积大，与平面纯铂电极相比，响应可提高8.3倍。所提出的生物传感器在葡萄糖定量分析中非常灵敏（22.4 μA cm⁻² mM⁻¹），线性范围为1 μmol L⁻¹至2 mmol L⁻¹，检测限为0.8 μmol L⁻¹。该生物传感器用于血清葡萄糖测定，获得了满意的结果。这项工作可能为安培池提供了一种可用于其他酶促FIA生物传感器的参考设计。
