Heo Soo-Jin, Jeon You-Jin
Marine Living Resources Research Department, Korea Ocean Research & Development Institute, Ansan 426-744, South Korea.
J Photochem Photobiol B. 2009 May 4;95(2):101-7. doi: 10.1016/j.jphotobiol.2008.11.011. Epub 2008 Dec 6.
Fucoxanthin is a carotenoid isolated from Sargassum siliquastrum and is considered to be one of major active compound of marine algae. In this study, we investigated and confirmed the protective effect of fucoxanthin on UV-B induced cell injury in human fibroblast via 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT), and comet assays. Intracellular ROS generated by exposure to UV-B radiation, which was significantly decreased by addition with various concentrations of fucoxanthin. Cell survival rate was increased with fucoxanthin pre-treated cells, which was reached around 81.47% at 100 microM, and the inhibitory effect of cell damage exhibited dose-dependent manner. Moreover, fucoxanthin having protective properties was demonstrated via Hoechst 33342/PI staining. Hence, on the basis of the above-mentioned studies, fucoxanthin has the ability to protect against oxidative stress induced by UV-B radiation and which might be applied to antioxidant and cosmeceutical industries.
岩藻黄质是一种从海带中分离出的类胡萝卜素，被认为是海藻的主要活性化合物之一。在本研究中，我们通过2',7'-二氯二氢荧光素二乙酸酯（DCFH-DA）、3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和彗星试验，研究并证实了岩藻黄质对紫外线B（UV-B）诱导的人成纤维细胞损伤的保护作用。暴露于UV-B辐射产生的细胞内活性氧（ROS），在添加不同浓度的岩藻黄质后显著降低。岩藻黄质预处理的细胞存活率增加，在100微摩尔时达到约81.47%，并且细胞损伤的抑制作用呈剂量依赖性。此外，通过Hoechst 33342/PI染色证明了岩藻黄质具有保护特性。因此，基于上述研究，岩藻黄质具有抵御UV-B辐射诱导的氧化应激的能力，并且可能应用于抗氧化和药妆行业。
