聚合嵌合体：将胚胎干细胞、二倍体和四倍体胚胎相结合。
Aggregation chimeras: combining ES cells, diploid, and tetraploid embryos.
作者信息
Tanaka Mika, Hadjantonakis Anna-Katerina, Vintersten Kristina, Nagy Andras
机构信息
Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada.
出版信息
Methods Mol Biol. 2009;530:287-309. doi: 10.1007/978-1-59745-471-1_15.
Abstract
During the past 40 years, mouse chimeras have served as invaluable tools for studying not only genetics but also embryonic development, and the path from undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more recently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.
摘要
在过去的40年里，小鼠嵌合体不仅成为研究遗传学的宝贵工具，还用于研究胚胎发育以及从未分化细胞群体到完全分化的功能细胞类型的转变过程。本章描述了植入前和植入后阶段胚胎中细胞定向分化和分化的早期事件。接下来，将重点讨论用于生产嵌合体的各种细胞类型和来源的最常用以及最近开发的应用。最后，将针对所涉及的每个步骤提供详细的方案和故障排除建议。
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