Kern Heidrun, Zevnik Branko
Department of Applied Genetics, ARTEMIS Pharmaceuticals, Cologne, Germany.
Methods Mol Biol. 2009;530:187-204. doi: 10.1007/978-1-59745-471-1_10.
A method is described to establish mouse embryonic stem cell (ESC) lines from hybrid and inbred strains of mice. Attention is paid not only to the methodology for isolation and culture but also to the validation of freshly derived lines, in order to be maintained for prolonged time without significant differentiation or karyotype instability, and to provide reproducible germline transmission in chimaeric mice.
本文描述了一种从小鼠杂交品系和近交品系建立小鼠胚胎干细胞（ESC）系的方法。不仅关注分离和培养方法，还关注新获得细胞系的验证，以便长时间维持而无明显分化或核型不稳定，并在嵌合小鼠中实现可重复的种系传递。
