Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Virology. 1991 Nov;185(1):464-7. doi: 10.1016/0042-6822(91)90800-q.
The nucleotide sequence of the RNA 3 of the peanut stunt virus strain J (PSV-J) was determined and compared with those of the cucumber mosaic virus strain Y (CMV-Y, subgroup I) and the tomato aspermy virus strain C (TAV-C) at both the nucleotide and protein levels. The RNA 3 of PSV-J consists of 2186 nucleotides and has two large open reading frames (ORFs). By analogy to other tripartite plant viruses, it is presumed that the first ORF (867 nucleotides) codes for the 3a protein and the second ORF (654 nucleotides) is the cistron of the coat protein which is expressed from subgenomic RNA 4 of 1010 nt residues. At the nucleotide level, PSV-J RNA 3 shows 60.6% homology to RNA 3 of CMV-Y and 66.3% to that of TAV-C, much lower than the value between the two CMV subgroups. For the 3a proteins there is 65.4% homology between PSV-J and CMV-Y and 70.2% homology between PSV-J and TAV-C. Although it has been known that PSV shows a distant serological relationship to CMV, the coat protein of PSV-J shows only 50% homology with that of CMV-Y.
测定了花生矮化病毒J株（PSV-J）RNA 3的核苷酸序列，并在核苷酸和蛋白质水平上与黄瓜花叶病毒Y株（CMV-Y，亚组I）和番茄不孕病毒C株（TAV-C）的核苷酸序列进行了比较。PSV-J的RNA 3由2186个核苷酸组成，有两个大的开放阅读框（ORF）。根据其他三分体植物病毒类推，推测第一个ORF（867个核苷酸）编码3a蛋白，第二个ORF（654个核苷酸）是由1010个核苷酸残基的亚基因组RNA 4表达的外壳蛋白的顺反子。在核苷酸水平上，PSV-J RNA 3与CMV-Y的RNA 3显示出60.6%的同源性，与TAV-C的RNA 3显示出66.3%的同源性，远低于两个CMV亚组之间的值。对于3a蛋白，PSV-J与CMV-Y之间有65.4%的同源性，PSV-J与TAV-C之间有70.2%的同源性。尽管已知PSV与CMV有较远的血清学关系，但PSV-J的外壳蛋白与CMV-Y的外壳蛋白仅显示出50%的同源性。
