Owen J, Shintaku M, Aeschleman P, Ben Tahar S, Palukaitis P
Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
J Gen Virol. 1990 Oct;71 ( Pt 10):2243-9. doi: 10.1099/0022-1317-71-10-2243.
The nucleotide sequence of RNA 3 of two subgroup I strains of cucumber mosaic virus (CMV), Fny-CMV and M-CMV, was determined and compared at both the nucleic acid and protein level with the previously determined, corresponding (partial) sequences of RNA 3 of five other subgroup I strains: C-CMV, D-CMV, I17F-CMV, O-CMV and Y-CMV. Fny-CMV RNA 3 is composed of 2216 nucleotides (nt) and M-CMV RNA 3 2214 nt. Both RNAs contain two open reading frames, the 3a gene and the coat protein gene. These RNAs showed very little nucleotide sequence divergence, either from each other or from the five other subgroup I strains. The nucleotide sequence variation observed was two to 13 differences in the 120 to 123 nt 5' non-translated regions, six to 17 differences in the 840 nt 3a genes, two to 15 differences in the 296 to 299 nt intergenic regions, three to 25 differences in the 657 nt coat protein genes and two to 10 differences in the 299 to 303 nt 3' non-translated regions. Protein sequence similarity was also high, with one to four differences in the 279 amino acids of the 3a proteins and two to 13 differences in the 218 amino acids of the coat proteins. Limited nucleotide sequence variation among nine strains of CMV was also shown using an RNA protection assay and a probe specific for Fny-CMV RNA 3. The limited variation shown by RNA 3 of strains of CMV with different passage histories, isolated in different countries over a 50 year period, suggests that the maintenance of the highly conserved nucleotide sequence may be important for other viral RNA functions or interactions.
测定了黄瓜花叶病毒（CMV）两个亚组I毒株Fny-CMV和M-CMV的RNA 3的核苷酸序列，并在核酸和蛋白质水平上与其他五个亚组I毒株（C-CMV、D-CMV、I17F-CMV、O-CMV和Y-CMV）先前测定的RNA 3相应（部分）序列进行了比较。Fny-CMV RNA 3由2216个核苷酸（nt）组成，M-CMV RNA 3由2214个nt组成。两种RNA均包含两个开放阅读框，即3a基因和外壳蛋白基因。这些RNA彼此之间以及与其他五个亚组I毒株之间的核苷酸序列差异很小。观察到的核苷酸序列变异为：120至123 nt的5'非翻译区有2至13个差异，840 nt的3a基因有6至17个差异，296至299 nt的基因间隔区有2至15个差异，657 nt的外壳蛋白基因有3至25个差异，299至303 nt的3'非翻译区有2至10个差异。蛋白质序列相似性也很高，3a蛋白的279个氨基酸中有1至4个差异，外壳蛋白的218个氨基酸中有2至13个差异。使用RNA保护试验和针对Fny-CMV RNA 3的探针也显示了CMV九个毒株之间有限的核苷酸序列变异。在50年期间于不同国家分离的具有不同传代历史的CMV毒株的RNA 3所显示的有限变异表明，高度保守的核苷酸序列的维持可能对其他病毒RNA功能或相互作用很重要。
