Dai Zhong-W, Zou Xiang-H, Chen Guo-Qiang
Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong, China.
Biomaterials. 2009 Jun;30(17):3075-83. doi: 10.1016/j.biomaterials.2009.02.015. Epub 2009 Mar 6.
An injectable implant system that immediately forms a film around the injection site of an animal was successfully developed by dissolving microbial polyester poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) in not harmful organic solvents including N-methyl pyrrolidone (NMP), dimethylacetamide (DMAC), 1,4-dioxane (DIOX), dimethyl sulfoxide (DMSO) and 1,4-butanolide (BL), respectively. The formation of the PHBHHx film was the result of contact between aqueous body fluids and the amphiphilic PHBHHx solvents, leading to the controllable precipitation (film formation) of PHBHHx around the contact site. The resultant PHBHHx film assumed the shapes of its surrounding cavities. The resulting porous PHBHHx film was not favorable for attachment of Human Embryo Lung Fibroblast (HELF) cells. As a consequence, the fibroblasts cultured on the PHBHHx film exhibited a spheroid-like morphology. It was found that hydrophilicity of the PHBHHx film prepared using the above technique was significantly reduced compared with the poly(lactic acid) (PLA) film prepared for the same purpose and a PHBHHx film prepared from chloroform casting. This reduced hydrophilicity explains the poor attachment of fibroblast cells to the injectable PHBHHx film, suggesting that the PHBHHx injectable implant system can be developed as a tissue adhesion prevention film for surgical operations.
通过将微生物聚酯聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）分别溶解于包括N-甲基吡咯烷酮（NMP）、二甲基乙酰胺（DMAC）、1,4-二氧六环（DIOX）、二甲基亚砜（DMSO）和1,4-丁内酯（BL）在内的无害有机溶剂中，成功开发出一种可在动物注射部位立即形成薄膜的可注射植入系统。PHBHHx薄膜的形成是由于体液与两亲性PHBHHx溶剂接触的结果，导致PHBHHx在接触部位周围可控沉淀（形成薄膜）。所得的PHBHHx薄膜呈现其周围腔隙的形状。所得的多孔PHBHHx薄膜不利于人胚肺成纤维细胞（HELF）的附着。因此，在PHBHHx薄膜上培养的成纤维细胞呈现出类球体形态。研究发现，与采用相同方法制备的聚乳酸（PLA）薄膜以及通过氯仿浇铸制备的PHBHHx薄膜相比，利用上述技术制备的PHBHHx薄膜的亲水性显著降低。亲水性的降低解释了成纤维细胞与可注射PHBHHx薄膜的附着性较差的原因，这表明PHBHHx可注射植入系统可被开发用作外科手术的组织粘连预防薄膜。
