Schröder C H, Kaerner H C, Munk K, Darai G
Intervirology. 1977;8(3):164-71. doi: 10.1159/000148891.
Rat embryo fibroblasts were abortively infected with various stocks of herpes simplex virus type 1 strain ANG at 42 degrees. In uninfected controls all of the cells died during an 8-day incubation period at the elevated temperature, whereas varying numbers of cells in the infected cultures survived and formed colonies during subsequent incubation for 3--4 weeks at 37 degrees. All of the survivors appeared to be morphologically transformed. Two types of survivors, epithelial- and spindle-like cells, which occurred at a ratio of approximately 1:1 in all assays, could be distinguished. The observed survival rates increased from about 1 x 10-7 to 3 x 10-5, corresponding to increasing fractions (0--50%) of a defective genotype present in the infecting virus stocks. The individual survival rates do not depend exclusively on the quantity of defective virions. The existence of different subtypes of defective genomes as a further parameter is discussed.
将1型单纯疱疹病毒ANG株的不同毒株在42℃下对大鼠胚胎成纤维细胞进行流产感染。在未感染的对照中，所有细胞在高温下8天的培养期内全部死亡，而在感染培养物中，不同数量的细胞存活下来，并在随后37℃下培养3 - 4周期间形成集落。所有存活细胞在形态上似乎都发生了转化。可以区分出两种类型的存活细胞，即上皮样细胞和梭形细胞，在所有实验中它们出现的比例约为1:1。观察到的存活率从约1×10⁻⁷增加到3×10⁻⁵，这与感染病毒株中存在的缺陷基因型的比例增加（0 - 50%）相对应。个体存活率并不完全取决于缺陷病毒粒子的数量。文中讨论了作为另一个参数的缺陷基因组不同亚型的存在情况。
