Szalanski A L, Taylor D B, Mullin P G
J Nematol. 2000 Jun;32(2):229-33.
DNA sequence analysis was used to characterize the nuclear ribosomal DNA ITS1 region and a portion of the COII and 16S rDNA genes of the mitochondrial genome from Steinernema entomopathogenic nematodes. Nuclear ITS1 nucleotide divergence among seven Steinernema spp. ranged from 6 to 22%, and mtDNA divergence among five species ranged from 12 to 20%. No intraspecific variation was observed among three S. feltiae strains. Phylogenetic analysis of both nuclear and mitochondrial DNA sequences confirms the existing morphological relationships of several Steinernema species. Both the rDNA ITS1 and mtDNA sequences were useful for resolving relationships among Steinernema taxa.
利用DNA序列分析对昆虫病原斯氏线虫属线虫的核糖体DNA ITS1区域以及线粒体基因组的部分COII和16S rDNA基因进行了特征分析。七个斯氏线虫属物种之间的核ITS1核苷酸差异范围为6%至22%，五个物种之间的线粒体DNA差异范围为12%至20%。在三种费氏斯氏线虫菌株中未观察到种内变异。对核DNA和线粒体DNA序列的系统发育分析证实了几种斯氏线虫属物种现有的形态学关系。rDNA ITS1和线粒体DNA序列都有助于解析斯氏线虫属分类群之间的关系。
