Kamran Muhammad, Javed Nazir, Ullah Ihsan, Nazir Shahid, Jamil Shakra, Iqbal Muhammad Zafar, Abbas Huma, Khan Sajid Aleem, Ehetisham Ul Haq Muhammad
Plant Pathology Research Institute, Ayub Agricultural Research Institute Faisalabad, 38000, Punjab, Pakistan.
Department of Plant Pathology, University of Agriculture Faisalabad, 38040, Punjab, Pakistan.
Plant Pathol J. 2019 Feb;35(1):51-62. doi: 10.5423/PPJ.OA.11.2017.0253. Epub 2019 Feb 1.
A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (10 and 10 spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified with the highest frequency (52.6%) followed by (36.8%) and (10.5%). The sizes of PCR products were 1.7 kb for and populations while populations of produced 1.1 kb fragment. The digestion with I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra- genetic variability exist in RKN populations which have better encumbrance with .
当PP - 3和PP - J在两种不同温度（25±2°C和30±2°C）和浓度（10和10孢子/毫升）下对116个根结线虫（RKN）种群进行抑制时，观察到了很大的可变反应。PCR反应扩增了不同RKN物种线粒体基因组中细胞色素氧化酶亚基II基因（COII）和rRNA基因大亚基（lrRNA）之间的基因间隔区。引物C2F3和1108的识别频率最高（52.6%），其次是（36.8%）和（10.5%）。对于和种群，PCR产物的大小为1.7 kb，而种群产生1.1 kb的片段。用I进行酶切在1.5%琼脂糖凝胶上产生了三种不同的片段长度模式。从当前研究得出结论，RKN种群中存在遗传变异性，其对具有更好的抑制作用。
