Sastre-Garau X, Loste M N, Vincent-Salomon A, Favre M, Mouret E, de la Rochefordiere A, Durand J C, Tartour E, Lepage V, Charron D
Department of Pathology, Institut Curie, Paris, France.
Int J Cancer. 1996 Jun 21;69(3):159-64. doi: 10.1002/(SICI)1097-0215(19960621)69:3<159::AID-IJC1>3.0.CO;2-Z.
Specific types of human papillomaviruses (HPV) are associated with most cases of pre-invasive and invasive neoplasia of the uterine cervix. HLA phenotype influences susceptibility and resistance to viral infections and may therefore influence the course of HPV-associated tumors. Some data suggest that specific HLA class-II alleles may be associated with protection from or susceptibility to papillomavirus-associated lesions, but these results are still controversial. Using molecular probes, we looked for associations between specific DQA1, DQB1, DRB1 HLA class-II alleles, HPV types and cervical cancer. The analysis was performed on a population of 126 patients with invasive cervical cancer. For HLA typing, 165 healthy individuals were taken as controls. The DRB1 1301/02 allele frequency significantly decreased in patients (11%) as compared to controls (29%). This difference in frequency was dependent on the HPV-positive status of tumors and was no longer significant in the group of HPV-negative lesions. The same trends were observed with the DRB1 1301/02-DQA1 0103-DQB1 0603 haplotype frequency. An increase in the frequency of the DRB1 1401/07 and DRB1 03 alleles was observed in patients under 40. Contrary to what has been reported in the literature, no increase in the DRB1 15 allele frequency was observed in our series and only a slight increase in the DQB1 03 frequency was found in patients (70%) compared to controls (58%). In our study, no positive correlations between cervical cancer in Frenchwomen and specific HLA DR-DQ haplotypes has been found. In contrast, a negative correlation between DRB1 1301/ 02 alleles and HPV-positive tumors has been observed. This may suggest a protective effect of DR13 against HPV-associated lesions of the cervix.
特定类型的人乳头瘤病毒（HPV）与大多数子宫颈癌前病变和浸润性肿瘤病例相关。HLA表型会影响对病毒感染的易感性和抵抗力，因此可能会影响HPV相关肿瘤的病程。一些数据表明，特定的HLA-II类等位基因可能与对乳头瘤病毒相关病变的保护或易感性有关，但这些结果仍存在争议。我们使用分子探针，寻找特定的DQA1、DQB1、DRB1 HLA-II类等位基因、HPV类型与宫颈癌之间的关联。分析针对126例浸润性宫颈癌患者群体进行。对于HLA分型，选取165名健康个体作为对照。与对照组（29%）相比，患者中DRB1 1301/02等位基因频率显著降低（11%）。这种频率差异取决于肿瘤的HPV阳性状态，在HPV阴性病变组中不再显著。DRB1 1301/02-DQA1 0103-DQB1 0603单倍型频率也观察到相同趋势。在40岁以下患者中，观察到DRB1 1401/07和DRB1 03等位基因频率增加。与文献报道相反，在我们的系列研究中未观察到DRB1 15等位基因频率增加，且与对照组（58%）相比，患者中仅发现DQB1 03频率略有增加（70%）。在我们的研究中，未发现法国女性宫颈癌与特定HLA DR-DQ单倍型之间存在正相关。相反，观察到DRB1 1301/02等位基因与HPV阳性肿瘤之间存在负相关。这可能表明DR13对子宫颈HPV相关病变具有保护作用。
