Di Domenico F, Perluigi M, Foppoli C, Blarzino C, Coccia R, De Marco F, Butterfield D A, Cini C
Department of Biochemical Sciences, University of Rome La Sapienza, P.le Aldo Moro, 5-00185 Rome, Italy.
Free Radic Res. 2009 Apr;43(4):365-75. doi: 10.1080/10715760902777329. Epub 2009 Mar 9.
UV solar radiation is the major environmental risk factor for malignant melanoma. A great effort is currently posed on the search of new compounds able to prevent or reduce UV-mediated cell damage. Ferulic acid is a natural compound recently included in the formulation of solar protecting dermatological products. The purpose of the present work was to assess whether its ethyl ester derivative, FAEE, could protect skin melanocytes from UV-induced oxidative stress and cell damage. Experiments on human melanocytes irradiated with UVB showed that FAEE treatment reduced the generation of ROS, with a net decrease of protein oxidation. FAEE treatment was accompanied by an induction of HSP70 and heme oxygenase, by a marked suppression of PARP activation and a significant suppression of apoptosis. Moreover FAEE prevented iNOS induction, thus suppressing the secondary generation of NO-derived oxidizing agents. FAEE may represent a potentially effective pharmacological approach to reduce UV radiation-induced skin damage.
紫外线太阳辐射是恶性黑色素瘤的主要环境风险因素。目前人们正在大力寻找能够预防或减少紫外线介导的细胞损伤的新化合物。阿魏酸是一种天然化合物，最近被纳入防晒皮肤科产品配方中。本研究的目的是评估其乙酯衍生物FAEE是否能保护皮肤黑素细胞免受紫外线诱导的氧化应激和细胞损伤。对经紫外线B照射的人黑素细胞进行的实验表明，FAEE处理可减少活性氧的产生，蛋白质氧化净减少。FAEE处理伴随着HSP70和血红素加氧酶的诱导，PARP激活的显著抑制和细胞凋亡的显著抑制。此外，FAEE可防止诱导型一氧化氮合酶的诱导，从而抑制一氧化氮衍生氧化剂的二次产生。FAEE可能是一种潜在有效的药理学方法，可减少紫外线辐射引起的皮肤损伤。
