Hodges Kurt B, Abdul-Karim Fadi W, Wang Mingsheng, Lopez-Beltran Antonio, Montironi Rodolfo, Easley Samantha, Zhang Shaobo, Wang Nancy, MacLennan Gregory T, Cheng Liang
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Appl Immunohistochem Mol Morphol. 2009 Jul;17(4):345-50. doi: 10.1097/PAI.0b013e318194d992.
Fibroadenoma (FA) and phyllodes tumor (PT) of the breast are biphasic tumors composed of variable proportions of epithelial and stromal cells. We identified a case of synchronous FA and PT in the same breast mass. Using laser capture microdissection, loss of heterozygosity analysis was performed on these components to gain potential insight into the histogenetic relationship between FA and PT. Genomic DNA was analyzed at 10 microsatellite loci by polymerase chain reaction. Both tumors showed allelic loss at D7S522. In addition, phylloid tumor showed allelic loss at TP53 and D22S264, not observed in FA. Our data suggest that FA and PT are clonally related and allelic loss at TP53 and D22S264 may be implicated in the progression of FA to phyllode tumor.
乳腺纤维腺瘤（FA）和叶状肿瘤（PT）是由不同比例的上皮细胞和基质细胞组成的双相肿瘤。我们发现了一例同一乳腺肿块中同时存在FA和PT的病例。利用激光捕获显微切割技术，对这些成分进行杂合性缺失分析，以深入了解FA和PT之间的组织发生关系。通过聚合酶链反应在10个微卫星位点分析基因组DNA。两种肿瘤在D7S522均显示等位基因缺失。此外，叶状肿瘤在TP53和D22S264显示等位基因缺失，而在FA中未观察到。我们的数据表明，FA和PT是克隆相关的，TP53和D22S264的等位基因缺失可能与FA进展为叶状肿瘤有关。
