Sharon E, Spiegel Y
J Nematol. 1996 Mar;28(1):124-8.
Various fluorescent conjugated lectins have been used for the detection of glycoconjugates on nematode surfaces under light microscopy. Several problems have been experienced with these reagents including penetration of the cuticle by fluorescent lectins, non-glycoconjugate specificity, strong nematode autofluorescence at the emission wavelength of the fluorescent dye, and prevention of persistent visualization due to rapid quenching of the fluorescent components. Gold-conjugated reagents combined with silver enhancement alleviated these difficulties when working with three phytonematode species (Heterodera avenae, H. latipons, and Meloidogyne javanica) and two entomopathogenic species (Steinernema carpocapsae and S. glaseri) under light-microscopy visualization of binding by fluorescent lectins and neoglycoproteins. Moreover, gold-conjugated reagents resulted in stable bindings that enabled long-term observations.
各种荧光共轭凝集素已被用于在光学显微镜下检测线虫表面的糖缀合物。使用这些试剂时遇到了几个问题，包括荧光凝集素穿透角质层、非糖缀合物特异性、线虫在荧光染料发射波长处强烈的自发荧光，以及由于荧光成分的快速淬灭而无法持续观察。当在光学显微镜下观察荧光凝集素和新糖蛋白的结合时，与银增强相结合的金共轭试剂缓解了使用三种植物寄生线虫（燕麦孢囊线虫、宽孢囊线虫和爪哇根结线虫）和两种昆虫病原线虫（小卷蛾斯氏线虫和格氏斯氏线虫）时遇到的这些困难。此外，金共轭试剂产生了稳定的结合，能够进行长期观察。
