Davis E L, Kaplan D T, Permar T A, Dickson D W, Mitchell D J
J Nematol. 1988 Oct;20(4):609-19.
Fluorescent conjugates of the lectins soybean agglutinin (SBA), Concanavalin A (Con A), wheat germ agglutinin (WGA), Lotus tetragonolobus agglutinin (LOT), and Limulus polyphemus agglutinin (LPA) bound primarily to amphidial openings and amphidial secretions of viable, preinfective second-stage juveniles (J2) of Meloidogyne incognita races 1 and 3 (Mil, Mi3) and M. javanica (Mj). No substantial difference in fluorescent lectin binding was observed among the populations examined. Binding of only LOT and LPA were inhibited in the presence of 0.1 M competitive sugar. Structural differences in amphidial carbohydrate complexes among populations of Mi 1, Mi3, and Mj were revealed by glycohydrolase treatment of preinfective J2 and subsequent labeling with fluorescent lectins. A quantitative microfiltration enzyme-linked lectin assay revealed previously undetected differences in lectin binding to nonglycohydrolase-treated J2. Freinfective J2 of Mj bound the greatest amount of SBA, LOT, and WGA, whereas J2 of Mil bound the most LPA.
凝集素大豆凝集素（SBA）、伴刀豆球蛋白A（Con A）、麦胚凝集素（WGA）、四角豆凝集素（LOT）和鲎试剂凝集素（LPA）的荧光缀合物主要结合到南方根结线虫1号和3号生理小种（Mi1、Mi3）以及爪哇根结线虫（Mj）的存活、感染前第二阶段幼虫（J2）的双感器孔和双感器分泌物上。在所检测的群体中，未观察到荧光凝集素结合存在实质性差异。在存在0.1M竞争性糖的情况下，仅LOT和LPA的结合受到抑制。通过对感染前J2进行糖水解酶处理并随后用荧光凝集素标记，揭示了Mi1、Mi3和Mj群体间双感器碳水化合物复合物的结构差异。定量微滤酶联凝集素测定揭示了之前未检测到的凝集素与未用糖水解酶处理的J2结合的差异。Mj的感染前J2结合的SBA、LOT和WGA量最大，而Mi1的J2结合的LPA最多。
