National Environmental Engineering Research Institute, CSIR Complex, Chennai, 600113, India.
Appl Biochem Biotechnol. 2010 Feb;160(4):1241-53. doi: 10.1007/s12010-009-8592-1. Epub 2009 Mar 10.
Removal of azo dyes from effluent generated by textile industries is rather difficult. Azo dyes represent a major class of synthetic colorants that are mutagenic and carcinogenic. Pseudomonas aeruginosa grew well in the presence of Remazol Orange (RO) and was able to decolorize and degrade it. In the present study, the decolorization and degradation efficiency using single culture P. aeruginosa with RO and textile wastewaters is studied. The elucidation of decolorization pathway for P. aeruginosa is of special interest. The degradation pathway and the metabolic products formed during the degradation were also predicted with the help of high performance liquid chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy analysis. The data show the cleavage of the azo dye RO to form both methyl metanilic acid and 4-aminobenzoic acid after decolorization and finally to oxidation forms benzoic acid, alkenes, aldehydes, and alkynes. The organism was able to decolorize the dye RO and wastewater effectively to the maximum of 82.4% and 62%, respectively.
从纺织工业产生的废水中去除偶氮染料相当困难。偶氮染料是一类主要的合成着色剂，具有致突变性和致癌性。铜绿假单胞菌在Remazol Orange（RO）的存在下生长良好，并能够对其进行脱色和降解。在本研究中，使用单一培养的铜绿假单胞菌和纺织废水研究了对 RO 的脱色和降解效率。铜绿假单胞菌的脱色途径的阐明特别有趣。借助高效液相色谱、傅里叶变换红外光谱和核磁共振波谱分析，还预测了降解过程中的降解途径和形成的代谢产物。数据表明，偶氮染料 RO 在脱色后分解为甲基间苯二胺和 4-氨基苯甲酸，最后氧化形成苯甲酸、烯烃、醛和炔烃。该生物能够有效地将染料 RO 和废水脱色，最大程度分别为 82.4%和 62%。
