de Farias Vanessa, Maranho Leila Teresinha, de Vasconcelos Eliane Carvalho, da Silva Carvalho Filho Marco Aurélio, Lacerda Luiz Gustavo, Azevedo Jayme Augusto Menegassi, Pandey Ashok, Soccol Carlos Ricardo
Department of Biology Science, Universidade Positivo, Prof. Pedro Viriato Parigot de Souza Street, Curitiba, PR, Brazil.
Appl Biochem Biotechnol. 2009 Apr;157(1):10-22. doi: 10.1007/s12010-009-8531-1. Epub 2009 Mar 10.
This work aimed at investigating both the tolerance and the phytodegradation potential of Erythrina crista-galli L. in petroleum-contaminated soil. It consisted in analyzing E. crista-galli germination, surviving, growth, and development when cultivated at different contaminant concentrations and pollutant degradation rates. This specimen was selected because it presented a special behavior among others also exposed to petroleum in an accident that occurred in the Araucaria region (south of Brazil), resulting in a four-million-liter oil spill. The experiment was carried out in a greenhouse containing non-contaminated soil (NCS), vegetated contaminated soil (VCS), and non-vegetated contaminated soil (NVCS) at the following petroleum concentrations: 25 g kg(-1) (VCS-25), 50 g kg(-1) (VCS-50), and 75 g kg(-1) (VCS-75). After 60 days, the soil samples were analyzed by gas chromatography. Germination was more and more evident as higher petroleum concentrations were observed. The surviving rates of groups NCS, VCS-25, VCS-50, and VCS-75 were 64%, 70%, 61%, and 96%, respectively. The VCS group growth was reduced when compared to the control group (NCS). The individuals exposed to petroleum pollution presented differences in the anatomic structure of their roots when compared to the NCS group. It was observed that the petroleum degradation rate was higher for VCS group than for NVCS. E. crista-galli is potentially recommended for petroleum-contaminated soils because of its positive association in the presence of contamination.
这项工作旨在研究刺桐在石油污染土壤中的耐受性和植物降解潜力。它包括分析刺桐在不同污染物浓度和污染物降解率下种植时的发芽、存活、生长和发育情况。选择该样本是因为在南巴西南洋杉地区发生的一起事故中，它在其他同样暴露于石油的植物中表现出特殊行为，那次事故导致了400万升的石油泄漏。实验在温室中进行，温室中有未受污染的土壤（NCS）、植被覆盖的污染土壤（VCS）和无植被的污染土壤（NVCS），石油浓度分别为：25 g kg(-1)（VCS - 25）、50 g kg(-1)（VCS - 50）和75 g kg(-1)（VCS - 75）。60天后，通过气相色谱法对土壤样本进行分析。随着观察到的石油浓度升高，发芽情况越来越明显。NCS组、VCS - 25组、VCS - 50组和VCS - 75组的存活率分别为64%、70%、61%和96%。与对照组（NCS）相比，VCS组的生长受到了抑制。与NCS组相比，暴露于石油污染的个体其根部的解剖结构存在差异。观察到VCS组的石油降解率高于NVCS组。由于刺桐在污染环境中的积极表现，它有望被推荐用于石油污染土壤的修复。
