Lütteke Thomas, von der Lieth Claus W
German Cancer Research Center (DKFZ), Central Spectroscopic Department (B090), Heidelberg, Germany.
Methods Mol Biol. 2009;534:293-310. doi: 10.1007/978-1-59745-022-5_21.
The 3D structural data of glycoprotein or protein-carbohydrate complexes that are found in the Protein Data Bank (PDB) are an interesting data source for glycobiologists. Unfortunately, carbohydrate components are difficult to find with the means provided by the PDB. The GLYCOSCIENCES.de internet portal offers a variety of tools and databases to locate and analyze these structures. This chapter describes how to find PDB entries that feature a specific carbohydrate structure and how to locate carbohydrate residues in a 3D structure file and to check their consistency. In addition to this, methods to statistically analyze torsion angles and the abundance of amino acids both in the neighborhood of glycosylation sites and in the spatial vicinity of non-covalently bound carbohydrate chains are summarized.
蛋白质数据库(PDB)中发现的糖蛋白或蛋白质 - 碳水化合物复合物的三维结构数据,对糖生物学研究者来说是一个有趣的数据源。不幸的是,利用PDB提供的手段很难找到碳水化合物成分。GLYCOSCIENCES.de互联网门户网站提供了各种工具和数据库来定位和分析这些结构。本章介绍了如何找到具有特定碳水化合物结构的PDB条目,以及如何在三维结构文件中定位碳水化合物残基并检查其一致性。除此之外,还总结了对扭转角以及糖基化位点附近和非共价结合碳水化合物链空间附近氨基酸丰度进行统计分析的方法。