采用柯里拉京作为植物化学标志物，验证用于标准化叶下珠（草药及商业提取物）的高效液相色谱法。

Validated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical marker.

作者信息

Colombo Renata, de L Batista Andrea N, Teles Helder L, Silva Geraldo H, Bomfim Giovani C C, Burgos Rosilene C R, Cavalheiro Alberto J, da Silva Bolzani Vanderlan, Silva Dulce Helena S, Pelícia Clícia R, Guimarães Fabiana M, Heimberg Maria Célia H

机构信息

Instituto de Química, Universidade Estadual Paulista, Araraquara, SP, Brazil.

出版信息

Biomed Chromatogr. 2009 Jun;23(6):573-80. doi: 10.1002/bmc.1155.

DOI:10.1002/bmc.1155

PMID:19277954

Abstract

Phyllanthus niruri L., commonly known in Brazil as 'quebra-pedra', has long been used in the treatment of diverse diseases and especially urolithiasis. The therapeutic effects of P. niruri are attributed to various compounds present in the plant, including the hydrolysable tannin corilagin. In the present study, high-performance liquid chromatography (HPLC-/PAD) profiles of leaves and commercial extracts of P. niruri were examined and three compounds, found to be present in all of the samples studied, were isolated by open column chromatography over C18)silica gel followed by preparative HPLC. These compounds were identified by nuclear magnetic resonance as corilagin, rutin and ethyl 3,4,5-trihydroxybenzoate. Corilagin, which has been proposed as a phytochemical marker for P. niruri, was employed as an external standard in the development and validation of a rapid and efficient qualitative and quantitative HPLC assay for the analyte. The method may be applied in the standardization of herbs and phytomedicines commercialized in Brazil as quebra-pedra.

摘要

叶下珠，在巴西通常被称为“quebra-pedra”，长期以来一直用于治疗各种疾病，尤其是尿路结石。叶下珠的治疗效果归因于该植物中存在的各种化合物，包括可水解单宁柯里拉京。在本研究中，检测了叶下珠叶片和商业提取物的高效液相色谱（HPLC-PAD）图谱，并通过在C18硅胶上进行开放柱色谱，然后进行制备型HPLC，从所有研究样品中分离出三种化合物。通过核磁共振鉴定这些化合物为柯里拉京、芦丁和3,4,5-三羟基苯甲酸乙酯。柯里拉京已被提议作为叶下珠的植物化学标志物，在开发和验证一种快速有效的分析物定性和定量HPLC测定法时用作外标。该方法可应用于巴西作为quebra-pedra商业化的草药和植物药的标准化。