Zheng Zhi-Zhong, Chen Liang-Hua, Liu Shao-Song, Deng Yuan, Zheng Guo-Hua, Gu Yue, Ming Yan-Lin
Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China; College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.
Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China.
Biomed Res Int. 2016;2016:9729275. doi: 10.1155/2016/9729275. Epub 2016 Sep 29.
L., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. However, the antitumor components in have not been reported. In order to verify the antitumor components of and the plants which have the high content of these components, we isolated the antitumor components with bioguided fraction and isolation, by different chromatographic methods from the ethyl acetate fraction of , and identified them as ethyl brevifolincarboxylate and corilagin by H-NMR, C-NMR, 2D-NMR, and mass spectrometric analyses. Cell cytotoxicity assays showed that corilagin has broad-spectrum antitumor activity, a better antitumor potential, and lower toxicity in normal cells. Besides, the coefficient of drug interaction (CDI) of 10 M corilagin and 20 M cDDP reached up to 0.77, which means corilagin can promote the antitumor activity of cDDP. Furthermore, by the extensive screening among 10 species of plants reported to contain corilagin, we found that Lour. has the maximum content of corilagin. In conclusion, corilagin is the major active antitumor composition in L. on HCC cells and has high content in .
L.是一种著名的药用植物，在全球范围内被用作民间抗肿瘤药物。然而，其抗肿瘤成分尚未见报道。为了验证L.的抗肿瘤成分以及含有这些成分高含量的植物，我们采用生物活性导向分离法，通过不同的色谱方法从L.的乙酸乙酯部位分离出抗肿瘤成分，并通过氢核磁共振（H-NMR）、碳核磁共振（C-NMR）、二维核磁共振（2D-NMR）和质谱分析将其鉴定为短叶苏木酚酸乙酯和柯里拉京。细胞毒性试验表明，柯里拉京具有广谱抗肿瘤活性、较好的抗肿瘤潜力且对正常细胞毒性较低。此外，10μM柯里拉京与20μM顺铂（cDDP）的药物相互作用系数（CDI）高达0.77，这意味着柯里拉京可促进顺铂的抗肿瘤活性。此外，通过对报道含有柯里拉京的10种植物进行广泛筛选，我们发现Lour.中柯里拉京的含量最高。综上所述，柯里拉京是L.对肝癌细胞的主要活性抗肿瘤成分，且在Lour.中含量很高。
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