Meyer Markus, Vilardell Josep
Gene Regulation Program, Centre de Regulació Genòmica, Dr Aiguader 88, Barcelona, Spain.
Brief Funct Genomic Proteomic. 2009 Jan;8(1):60-7. doi: 10.1093/bfgp/elp002. Epub 2009 Mar 11.
Removal of introns during pre-mRNA splicing is a critical process in gene expression, and understanding its control at both single-gene and genomic levels is one of the great challenges in Biology. Splicing takes place in a dynamic, large ribonucleoprotein complex known as the spliceosome. Combining Genetics and Biochemistry, Saccharomyces cerevisiae provides insights into its mechanisms, including its regulation by RNA-protein interactions. Recent genome-wide analyses indicate that regulated splicing is broad and biologically relevant even in organisms with a relatively simple intronic structure, such as yeast. Furthermore, the possibility of coordination in splicing regulation at genomic level is becoming clear in this model organism. This should provide a valuable system to approach the complex problem of the role of regulated splicing in genomic expression.
前体mRNA剪接过程中内含子的去除是基因表达中的一个关键过程，而在单基因和基因组水平上理解其调控是生物学领域的重大挑战之一。剪接发生在一种动态的、大型核糖核蛋白复合体即剪接体中。通过结合遗传学和生物化学方法，酿酒酵母为其机制提供了深入见解，包括其通过RNA-蛋白质相互作用进行的调控。最近的全基因组分析表明，即使在具有相对简单内含子结构的生物体（如酵母）中，受调控的剪接也很广泛且具有生物学相关性。此外，在这种模式生物中，基因组水平上剪接调控协调的可能性正变得清晰。这应该为解决受调控剪接在基因组表达中的作用这一复杂问题提供一个有价值的系统。
