Lecrenier N, Foury F, Goffeau A
Laboratoire de Biochimie Physiologique, Louvain-La-Neuve, Belgium.
Bioessays. 1998 Jan;20(1):1-5. doi: 10.1002/(SICI)1521-1878(199801)20:1<1::AID-BIES2>3.0.CO;2-Y.
The yeast two-hybrid system is a genetic method that detects protein-protein interactions. One application is the detection by library screening of new interactors of a protein of known function. In the August issue of Nature Genetics, Fromont-Racine et al. showed for the first time that the construction of the protein interaction map of a complex pathway, such as that of the mRNA splicing machinery, is now possible, because of the combination of recent technical improvements elaborated in several laboratories. With a yeast cell mating procedure that increases screen efficiency, they used their complex yeast genomic library of 5 x 10(6) clones to test 700 x 10(6) interactions against 15 proteins. They identified and classified 170 potential interactors, including approximately 70 proteins of previously unknown function. More than 25% of the interactors are probably biologically relevant. The achievements of Fromont-Racine et al. have opened the way to the systematic analysis of the protein interaction networks of the 6,000 open reading frames-yeast proteome. This task requires, however, automation of the library screens and creation of a two-hybrid library database.
酵母双杂交系统是一种检测蛋白质 - 蛋白质相互作用的遗传学方法。其一个应用是通过文库筛选来检测已知功能蛋白质的新相互作用蛋白。在《自然遗传学》八月刊中，弗罗蒙 - 拉辛等人首次表明，由于几个实验室近期技术改进的结合，构建复杂途径（如mRNA剪接机制）的蛋白质相互作用图谱现在成为可能。通过一种提高筛选效率的酵母细胞交配程序，他们利用其包含5×10⁶个克隆的复杂酵母基因组文库，针对15种蛋白质测试了700×10⁶次相互作用。他们鉴定并分类了170个潜在相互作用蛋白，其中包括约70个功能未知的蛋白质。超过25%的相互作用蛋白可能具有生物学相关性。弗罗蒙 - 拉辛等人的成果为对6000个开放阅读框（酵母蛋白质组）的蛋白质相互作用网络进行系统分析开辟了道路。然而，这项任务需要文库筛选的自动化以及创建一个双杂交文库数据库。
