Ren Lu-Jing, Huang He, Xiao Ai-Hua, Lian Min, Jin Li-Jing, Ji Xiao-Jun
College of Life Science and Pharmacy, Nanjing University of Technology, Gulou District, 210009 Nanjing, China.
Bioprocess Biosyst Eng. 2009 Oct;32(6):837-43. doi: 10.1007/s00449-009-0310-4. Epub 2009 Mar 12.
Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) at different fermentation stages. According to the analysis, a regulation strategy was proposed which reinforced acetyl-CoA and NADPH supply at a specific fermentation stage. DHA content of total fatty acids was increased from 35 to 60% by the addition of 4 g/L malic acid at the rapid lipid accumulation stage. Total lipid content also showed an apparent increase of 35% and reached 19 g/L when 40 mL ethanol/L was added at the late lipid accumulation stage.
通过检测裂殖壶菌属HX - 308在不同发酵阶段的ATP：柠檬酸裂解酶（EC 4.1.3.8）、苹果酸酶（EC 1.1.1.40）和葡萄糖 - 6 - 磷酸脱氢酶（EC 1.1.1.49）的酶活性，评估了该菌株中二十二碳六烯酸（DHA）的产量。根据分析结果，提出了一种调控策略，即在特定发酵阶段加强乙酰辅酶A和NADPH的供应。在快速脂质积累阶段添加4 g/L苹果酸后，总脂肪酸中的DHA含量从35%提高到了60%。在脂质积累后期添加40 mL乙醇/L时，总脂质含量也明显增加了35%，达到了19 g/L。
