Lin Jia-Xue, Wang Ying, Zhao Shao-Geng, Huang Bei-Chen, Zhang Yue, Wang Zhi-Qiang, Li Sheng-Jie, Liu Qian, Ma Yan, Ye Jing-Run, Wang Hai-Ying, Wang Zhi-Peng
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, China.
Department of Obstetrics and Gynecology, Cheeloo College of Medicine, Qilu Hospital (Qingdao), Shandong University, Qingdao, Shandong, P.R. China.
World J Microbiol Biotechnol. 2025 Jul 30;41(8):290. doi: 10.1007/s11274-025-04511-7.
Aurantiochytrium limacinum is recognized as an ideal candidate to produce docosahexaenoic acid (DHA). However, elevating the biomass and lipid yield on a large scale is still a challenge. In this study, a simple and feasible strategy for enhancing DHA accumulation based on Laminaria japonica hydrolysate (LPH) addition has been developed. The addition of 40 mL/L LPH enhanced the accumulation of biomass and lipid of A. limacinum by 31.48% and 49.48%, respectively. Meanwhile, the DHA production was increased by 40.78%. By investigating the effects on DHA and biomass accumulation hormones in LPH, gibberellin 3, abscisic acid, and salicylic acid were characterized as the key factor. With the addition of LPH, the upregulated genes were enriched in pathways including fatty acid biosynthesis, metabolism, and the tricarboxylic acid cycle. In particular, key genes involved in fatty acid synthesis, such as ACC1 and FAS2, were significantly upregulated. In this study, LPH was identified as a novel additive with practical application value due to its readily available and low-cost raw ingredients.
纤细金藻被认为是生产二十二碳六烯酸（DHA）的理想候选者。然而，大规模提高生物量和脂质产量仍然是一个挑战。在本研究中，开发了一种基于添加海带水解物（LPH）来提高DHA积累的简单可行策略。添加40 mL/L的LPH分别使纤细金藻的生物量和脂质积累提高了31.48%和49.48%。同时，DHA产量提高了40.78%。通过研究LPH中对DHA和生物量积累激素的影响，赤霉素3、脱落酸和水杨酸被确定为关键因素。随着LPH的添加，上调基因富集在脂肪酸生物合成、代谢和三羧酸循环等途径中。特别是，参与脂肪酸合成的关键基因，如ACC1和FAS2，显著上调。在本研究中，LPH因其原料易得且成本低而被确定为具有实际应用价值的新型添加剂。
