Seitz G, Thelsinger B, Tomasetto G, Rio M C, Chambon P, Blin N, Welter G
Institut für Allgem, Pathologie, Homburg, Germany.
Am J Gastroenterol. 1991 Oct;86(10):1491-4.
Expression of pS2 (an estrogen-induced gene isolated from breast carcinoma MCF7 cells) and of the human spasmolytic peptide (hSP) gene was analyzed in 21 human biliary tract and gallbladder carcinomas and 16 non-neoplastic gallbladders at the protein level (immunochemistry) and, in a series of these cases, at the RNA level (Northern blots). Eighteen carcinomas and 14 non-neoplastic mucosae with inflammatory alterations showed pS2 activity by immunostaining or Northern blotting. In addition, in samples with pS2 expression, hSP RNA was demonstrated. In the corresponding non-neoplastic and healthy mucosae, pS2 was negative, as judged by immunostaining. Northern blots showed weak (basal) level of activity for pS2 and hSP. The genes' increased expression in correlation to inflammatory and neoplastic processes, by now observed in several carcinomas and idiopathic inflammatory bowel disease, hints to their essential role in such diseases.
在21例人胆管和胆囊癌以及16例非肿瘤性胆囊中，通过蛋白质水平（免疫化学）并在其中一部分病例中通过RNA水平（Northern印迹法）分析了pS2（一种从乳腺癌MCF7细胞中分离出的雌激素诱导基因）和人解痉肽（h SP）基因的表达。18例癌和14例有炎性改变的非肿瘤性黏膜通过免疫染色或Northern印迹法显示出pS2活性。此外，在有pS2表达的样本中，证实存在hSP RNA。通过免疫染色判断，在相应的非肿瘤性和健康黏膜中，pS2呈阴性。Northern印迹法显示pS2和hSP的活性处于较弱（基础）水平。目前在几种癌症和特发性炎症性肠病中观察到这些基因的表达与炎症和肿瘤过程相关增加，这提示它们在这些疾病中起重要作用。
