Albert Genevieve C, McNamee James P, Marro Leonora, Bellier Pascale V, Prato Frank S, Thomas Alex W
Bioelectromagnetics, Lawson Health Research Institute, Department of Medical Biophysics, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario.
Int J Radiat Biol. 2009 Feb;85(2):144-52. doi: 10.1080/09553000802641169.
To investigate the extent of damage in nucleated cells in peripheral blood of healthy human volunteers exposed to a whole-body 60 Hz, 200 microT magnetic field.
In this study, 10 male and 10 female healthy human volunteers received a 4 h whole-body exposure to a 200 microT, 60 Hz magnetic field. In addition, five males and five females were treated in a similar fashion, but were exposed to sham conditions. For each subject, a blood sample was obtained prior to the exposure period and aliquots were used as negative- (pre-exposure) and positive- [1.5 Gray (Gy) (60)Cobalt ((60)Co) gamma-irradiation] controls. At the end of the 4 h exposure period, a second blood sample was obtained. The extent of DNA damage was assessed in peripheral human blood leukocytes from all samples using the alkaline comet assay. To detect possible clastogenic effects, the incidence of micronuclei was assessed in phytohemagglutinin (PHA)-stimulated lymphocytes using the cytokinesis-block micronucleus assay.
There was no evidence of either increased DNA damage, as indicated by the alkaline comet assay, or increased incidence of micronuclei (MN) in the magnetic field exposed group. However, an in vitro exposure of 1.5 Gy gamma-irradiation caused a significant increase in both DNA damage and MN induction.
This study found no evidence that an acute, whole-body exposure to a 200 microT, 60 Hz magnetic field for 4 hours could cause DNA damage in human blood.
研究健康人类志愿者全身暴露于60赫兹、200微特斯拉磁场后外周血中有核细胞的损伤程度。
在本研究中，10名男性和10名女性健康人类志愿者全身暴露于200微特斯拉、60赫兹的磁场中4小时。此外，5名男性和5名女性以类似方式接受处理，但暴露于假环境中。对于每个受试者，在暴露期之前采集血液样本，其等分试样用作阴性（暴露前）和阳性[1.5戈瑞（Gy）（60）钴（（60）Co）γ射线照射]对照。在4小时暴露期结束时，采集第二份血液样本。使用碱性彗星试验评估所有样本中人类外周血白细胞的DNA损伤程度。为了检测可能的致断裂效应，使用胞质分裂阻滞微核试验评估植物血凝素（PHA）刺激的淋巴细胞中的微核发生率。
碱性彗星试验表明，磁场暴露组既没有DNA损伤增加的证据，也没有微核（MN）发生率增加的证据。然而，1.5 Gyγ射线的体外照射导致DNA损伤和MN诱导均显著增加。
本研究没有发现证据表明急性全身暴露于200微特斯拉、60赫兹的磁场4小时会导致人类血液中的DNA损伤。
