Noetzel Jörn, Nonhoff Jörg, Bitter Kerstin, Wagner Jutta, Neumann Konrad, Kielbassa Andrej M
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Charité-Universitätsmedizin Berlin, Assmannshauser Str. 4-6, 14197 Berlin, Germany.
Am J Dent. 2009 Feb;22(1):14-8.
To evaluate the efficacy of Ca(OH)2, Er:YAG laser or gaseous ozone (either alone or combined with instrumentation and various irrigants) against Enterococcus faecalis in root canals.
180 extracted, human, single-rooted teeth were divided into four groups of 45 teeth each. In Group 1 root canal enlargement up to ISO-size 60 (MAF) was performed, whereas only initial shaping (MAF ISO-size 40) was carried out in Groups 2 to 4. After sterilization all teeth were inoculated with E. faecalis and incubated for 3 days, followed by evaluation of CFU. Subsequently, root canal enlargement up to ISO-size 60 was performed in Groups 2 to 4 using NaCl solution (0.9%) in Group 2, NaOCl (1%) in Group 3 and CHX (0.2%) in Group 4. Finally, each group of 45 teeth was subdivided into three groups (n= 15 each) either applying Ca(OH)2 for 7 days, using Er:YAG laser radiation for 30 seconds or gaseous ozone for 120 seconds, followed by final evaluation of CFU.
Both in Groups 1 and 2 the median reduction of bacteria after application of Ca(OH)2 (factor 10(4) each) and ozone (in Group 1: factor 5 x 10(3); in Group 2: factor 5 x 10(4)), respectively, was significantly higher than after Er:YAG laser treatment (factor 102 each, Mann-Whitney test). The antibacterial efficacy was significantly increased by the additional use of NaOCl or CHX as irrigants in all subgroups (Groups 3 and 4) compared to corresponding subgroups of Group 1 (Mann-Whitney test).
评估氢氧化钙、铒激光或气态臭氧（单独使用或与器械和各种冲洗剂联合使用）对根管内粪肠球菌的疗效。
180颗拔除的人单根牙被分为四组，每组45颗牙。第1组进行根管扩大至ISO 60号（MAF），而第2至4组仅进行初始预备（MAF ISO 40号）。消毒后，所有牙齿接种粪肠球菌并培养3天，随后评估菌落形成单位（CFU）。随后，第2至4组使用氯化钠溶液（0.9%）、次氯酸钠（1%）和洗必泰（0.2%）进行根管扩大至ISO 60号。最后，每组45颗牙再细分为三组（每组n = 15），分别应用氢氧化钙7天、铒激光照射30秒或气态臭氧120秒，随后再次评估CFU。
在第1组和第2组中，应用氢氧化钙（每组降低10⁴倍）和臭氧（第1组降低5×10³倍；第2组降低5×10⁴倍）后细菌数量的中位数减少均显著高于铒激光治疗后（每组降低10²倍，曼-惠特尼检验）。与第1组的相应亚组相比，在所有亚组（第3组和第4组）中额外使用次氯酸钠或洗必泰作为冲洗剂可显著提高抗菌效果（曼-惠特尼检验）。
