Acquadro Elena, Cabella Claudia, Ghiani Simona, Miragoli Luigi, Bucci Enrico M, Corpillo Davide
Laboratorio Integrato Metodologie Avanzate, Bioindustry Park Canavese S.p.A., Torino, Italy.
Anal Chem. 2009 Apr 1;81(7):2779-84. doi: 10.1021/ac900038y.
The present paper describes the detection of a magnetic resonance imaging (MRI) contrast agent by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS). The contrast agent was analyzed in both frozen and paraformaldehyde-fixed mouse livers explanted after its in vivo administration, and its identity was confirmed by fragmentation experiments. Moreover, a semiquantitative analysis was performed, evaluating its content in livers from mice sacrificed at different postadministration times. To the best of our knowledge, this is the first description of a MALDI-IMS analysis of MRI contrast agents and the first time that results obtained by MALDI-IMS are validated by both an in vivo (MRI) and an ex vivo (inductively coupled plasma atomic emission spectroscopy, ICP-AES) technique. Results shown in the present paper demonstrate the possibility of using MALDI-IMS for drug biodistribution analysis. Obviously, this application is particularly interesting in the case of unlabeled compounds, which cannot be detected by any of the other imaging techniques.
本文描述了通过基质辅助激光解吸电离成像质谱法（MALDI-IMS）检测磁共振成像（MRI）造影剂。在体内给药后取出的冷冻和多聚甲醛固定的小鼠肝脏中分析该造影剂，并通过碎片实验确认其身份。此外，进行了半定量分析，评估了在不同给药后时间处死的小鼠肝脏中其含量。据我们所知，这是对MRI造影剂进行MALDI-IMS分析的首次描述，也是首次通过体内（MRI）和体外（电感耦合等离子体原子发射光谱法，ICP-AES）技术验证MALDI-IMS获得的结果。本文所示结果证明了使用MALDI-IMS进行药物生物分布分析的可能性。显然，这种应用在未标记化合物的情况下特别有趣，因为其他任何成像技术都无法检测到这些化合物。
