活细胞动力学的双干涉通道定量相位显微镜术
Dual-interference-channel quantitative-phase microscopy of live cell dynamics.
作者信息
Shaked Natan T, Rinehart Matthew T, Wax Adam
机构信息
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA.
出版信息
Opt Lett. 2009 Mar 15;34(6):767-9. doi: 10.1364/ol.34.000767.
Abstract
We introduce and experimentally demonstrate a fast and accurate method for quantitative imaging of the dynamics of live biological cells. Using a dual-channel interferometric setup, two phase-shifted interferograms of nearly transparent biological samples are acquired in a single digital camera exposure and digitally processed into the phase profile of the sample. Since two interferograms of the same sample are acquired simultaneously, most of the common phase noise is eliminated, enabling the visualization of millisecond-scale dynamic biological phenomena with subnanometer optical path length temporal stability.
摘要
我们介绍并通过实验证明了一种用于对活生物细胞动力学进行定量成像的快速且准确的方法。使用双通道干涉测量装置，在单次数码相机曝光中获取几乎透明生物样品的两张相移干涉图，并对其进行数字处理以得到样品的相位分布。由于同时获取了同一样品的两张干涉图，大部分常见的相位噪声被消除，从而能够以亚纳米光程长度时间稳定性可视化毫秒级动态生物现象。
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