具有相移数字全息术的三维显微镜技术。

Three-dimensional microscopy with phase-shifting digital holography.

作者信息

Zhang T, Yamaguchi I

机构信息

Graduate Course of Science and Engineering, Saitama University, Urawa, Saitama 338-8570, Japan.

出版信息

Opt Lett. 1998 Aug 1;23(15):1221-3. doi: 10.1364/ol.23.001221.

DOI:10.1364/ol.23.001221

PMID:18087480

Abstract

We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

摘要

我们通过摄像机记录、相移分析和计算机重建，推导通过显微镜物镜从微观三维物体散射的光的复振幅，将相移数字全息术应用于显微镜。该方法无需机械移动，并能对重建图像进行灵活显示和定量评估。文中描述了图像形成理论及对标本的实验验证。