Gliko Olga, Brownell William E, Saggau Peter
Department of Otorhinolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
Opt Lett. 2009 Mar 15;34(6):836-8. doi: 10.1364/ol.34.000836.
We report a scheme for 2D standing-wave total-internal-reflection fluorescence microscopy. Standing-wave patterns are generated by two interfering beams coupled through the objective lens. Period, angular orientation, and phase of standing waves are controlled entirely by acousto-optic deflectors. The lateral resolution improvement of 100 nm is combined with an axial selectivity of <100 nm by utilizing an evanescent standing-wave pattern. This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass-water interface.
我们报道了一种用于二维驻波全内反射荧光显微镜的方案。驻波图案由通过物镜耦合的两束干涉光束产生。驻波的周期、角取向和相位完全由声光偏转器控制。通过利用倏逝驻波图案，横向分辨率提高了100纳米，并结合了小于100纳米的轴向选择性。该技术可以对玻璃 - 水界面附近的活生物样本中的亚分辨率结构进行实时成像。
