Kennedy G A, Coleman G J, Armstrong S M
Department of Psychology, La Trobe University, Bundoora, Victoria, Australia.
Am J Physiol. 1991 Oct;261(4 Pt 2):R819-27. doi: 10.1152/ajpregu.1991.261.4.R819.
The effect of daily restricted feeding (RF) on the circadian wheel-running rhythms of the kowari, Dasyuroides byrnei, was examined in two experiments. Kowaris were presented with a preferred food (determined in a pilot study) during a daily 2-h meal in the light period of a 14:10 light-dark (LD) cycle (expts 1 and 2), during constant dark (DD) immediately after termination of the LD cycle (expt 1), and during DD when kowaris were free running (expt 1). Results showed that 1) RF elicited anticipatory activity similar in duration and phasing to that observed in the rat; 2) cycles of meal-associated activity free ran for up to 6 days after the termination of RF; 3) activity persists at a phase near that of the former mealtime during periods of food deprivation; and 4) activity indicative of beating between two pacemakers occurred when feeding was restricted to the L period of LD cycles. Together these observations suggest that the activity rhythms of the kowari may be controlled by separate, but possibly coupled, light-entrainable and food-entrainable pacemakers, as are those of the rat.
在两项实验中，研究了每日定时限食（RF）对南澳伪沼鼠（Dasyuroides byrnei）昼夜节律性转轮行为的影响。在14:10光暗（LD）循环的光照期内，每天给南澳伪沼鼠2小时的进食时间，提供其偏爱的食物（在预实验中确定）（实验1和2）；在LD循环结束后立即进入持续黑暗（DD）环境时进行实验（实验1）；以及在南澳伪沼鼠自由活动的DD环境中进行实验（实验1）。结果表明：1）RF引发的预期活动在持续时间和相位上与在大鼠中观察到的相似；2）与进食相关的活动周期在RF结束后可自由运行长达6天；3）在食物剥夺期间，活动在接近先前进食时间的相位持续存在；4）当限食在LD循环的光照期进行时，出现了表明两个起搏器之间交替的活动。这些观察结果共同表明，南澳伪沼鼠的活动节律可能像大鼠一样，由独立但可能相互耦合的、可被光和食物调节的起搏器控制。
