Duthie Carol-Anne, Simm Geoff, Pérez-Enciso Miguel, Doeschl-Wilson Andrea, Kalm Ernst, Knap Pieter W, Roehe Rainer
Animal Breeding and Development, Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh, UK.
Genet Sel Evol. 2009 Mar 11;41(1):27. doi: 10.1186/1297-9686-41-27.
A QTL analysis of pig chromosome X (SSCX) was carried out using an approach that accurately takes into account the specific features of sex chromosomes i.e. their heterogeneity, the presence of a pseudoautosomal region and the dosage compensation phenomenon. A three-generation full-sib population of 386 animals was created by crossing Pietrain sires with a crossbred dam line. Phenotypic data on 72 traits were recorded for at least 292 and up to 315 F2 animals including chemical body composition measured on live animals at five target weights ranging from 30 to 140 kg, daily gain and feed intake measured throughout growth, and carcass characteristics obtained at slaughter weight (140 kg). Several significant and suggestive QTL were detected on pig chromosome X: (1) in the pseudoautosomal region of SSCX, a QTL for entire loin weight, which showed paternal imprinting, (2) closely linked to marker SW2456, a suggestive QTL for feed intake at which Pietrain alleles were found to be associated with higher feed intake, which is unexpected for a breed known for its low feed intake capacity, (3) at the telomeric end of the q arm of SSCX, QTL for jowl weight and lipid accretion and (4) suggestive QTL for chemical body composition at 30 kg. These results indicate that SSCX is important for physical and chemical body composition and accretion as well as feed intake regulation.
利用一种能准确考虑性染色体特殊特征（即其异质性、假常染色体区域的存在和剂量补偿现象）的方法，对猪的X染色体（SSCX）进行了数量性状基因座（QTL）分析。通过将皮特兰种公猪与一个杂交母系进行杂交，创建了一个由386只动物组成的三代全同胞群体。记录了至少292只、至多315只F2动物的72个性状的表型数据，包括在30至140千克五个目标体重下对活体动物测量的化学身体组成、整个生长过程中测量的日增重和采食量，以及在屠宰体重（140千克）时获得的胴体特征。在猪X染色体上检测到了几个显著和暗示性的QTL：（1）在SSCX的假常染色体区域，一个影响整个腰肉重量的QTL，表现出父本印记；（2）与标记SW2456紧密连锁，一个暗示性的采食量QTL，发现皮特兰等位基因与较高的采食量相关，这对于一个以低采食量能力著称的品种来说是出乎意料的；（3）在SSCX的q臂的端粒末端，影响颊肉重量和脂质沉积的QTL；（4）在30千克时影响化学身体组成的暗示性QTL。这些结果表明，SSCX对身体的物理和化学组成、沉积以及采食量调节很重要。
