Gates Maureen C, Nolan Thomas J
School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
J Parasitol. 2009 Oct;95(5):1213-4. doi: 10.1645/GE-2058.1. Epub 2009 Mar 13.
The sensitivity of fecal examination methods can be influenced by both technician error and methodology. In this analysis, we compared the results of 335 passive fecal flotation examinations performed on the feces of stray dogs by 3rd-yr veterinary students at the University of Pennsylvania, School of Veterinary Medicine, to the results obtained through zinc-sulfate centrifugation performed by the diagnostic parasitology laboratory on the same fecal samples. The students' passive flotation results agreed with the laboratory zinc-sulfate centrifugation for only 62.4% of samples. Students were able to diagnose 75.0% of Ancylostoma caninum cases, 71.4% of Toxocara canis cases, 54.2% of Trichuris vulpis cases, 26.7% of Cystoisospora spp. (C. ohioensis-like and C. canis) cases, and 14.7% of Giardia lamblia cases. There were also 70 instances where students reported the presence of parasites in the sample that were not diagnosed by zinc-sulfate centrifugation. Based on the overall study findings, passive fecal flotation examinations run in private practice could be missing up to 50.5% of infected dogs, due to either technician error or inherent limitations to the passive fecal flotation technique.
粪便检查方法的敏感性可能会受到技术人员误差和方法学的影响。在本分析中，我们将宾夕法尼亚大学兽医学院三年级兽医专业学生对流浪狗粪便进行的335次被动粪便浮选检查结果，与诊断寄生虫学实验室对相同粪便样本进行硫酸锌离心检查所获得的结果进行了比较。学生的被动浮选结果与实验室硫酸锌离心结果仅在62.4%的样本中一致。学生能够诊断出75.0%的犬钩虫病例、71.4%的犬弓首蛔虫病例、54.2%的狐毛首线虫病例、26.7%的等孢球虫属（俄亥俄等孢球虫样和犬等孢球虫）病例以及14.7%的贾第虫病例。此外，有70例学生报告样本中存在寄生虫，但硫酸锌离心检查未诊断出这些寄生虫。基于总体研究结果，由于技术人员误差或被动粪便浮选技术的固有局限性，私人诊所进行的被动粪便浮选检查可能会漏诊高达50.5%的感染犬。
