Uchôa Flávia Fernandes de Mendonça, Sudré Adriana Pittella, Macieira Daniel de Barros, Almosny Nádia Regina Pereira
Universidade Federal Fluminense, Faculdade de Medicina Veterinária, Niterói, Rio de Janeiro, Brazil.
Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, Rio de Janeiro, Brazil.
Rev Inst Med Trop Sao Paulo. 2017 Aug 24;59:e61. doi: 10.1590/S1678-9946201759061.
Giardia infection is a common clinical problem in humans and pets. The diagnosis of giardiasis is challenging as hosts intermittently excrete protozoan cysts in their feces. In the present study, we comparatively evaluated two methods of serial fecal sampling in humans, dogs, and cats from Rio de Janeiro, Brazil. The Faust et al. technique was used to examine fecal specimens collected in triplicate from 133 patients (52 humans, 60 dogs, and 21 cats). Specimens from 74 patients were received from the group assigned to carry out sampling on consecutive days - 34 humans, 35 dogs, and 5 cats, and specimens from 59 patients were received from the group assigned to carry out sampling on non-consecutive, separate days - 18 human beings, 25 dogs, and 16 cats. G. duodenalis cysts were found in stools of 30 individuals. Multiple stool sampling resulted in an increase in the number of samples that were positive for Giardia in both groups. The authors therefore conclude that multiple stool sampling increases the sensitivity of the Faust et al . technique to detect G. duodenalis cysts in samples from humans, cats and dogs.
贾第虫感染是人类和宠物中常见的临床问题。由于宿主会间歇性地在粪便中排出原生动物囊肿，贾第虫病的诊断具有挑战性。在本研究中，我们比较评估了巴西里约热内卢人类、狗和猫的两种连续粪便采样方法。使用福斯特等人的技术对133名患者（52名人类、60只狗和21只猫）的粪便标本进行了三次采集检查。74名患者的标本来自被分配在连续几天进行采样的组——34名人类、35只狗和5只猫，59名患者的标本来自被分配在非连续、分开的日子进行采样的组——18名人类、25只狗和16只猫。在30个人的粪便中发现了十二指肠贾第虫囊肿。多次粪便采样导致两组中贾第虫检测呈阳性的样本数量增加。因此，作者得出结论，多次粪便采样提高了福斯特等人的技术在检测人类、猫和狗样本中十二指肠贾第虫囊肿的敏感性。
