Departamento de Parasitologia, Instituto de Biociências, Unesp, Botucatu, SP, 18.618-000, Brazil.
Exp Parasitol. 2010 Oct;126(2):214-6. doi: 10.1016/j.exppara.2010.04.027. Epub 2010 May 7.
The aim of present study was to compare the efficiency of a commercial assay and two conventional methods for fecal concentration in detecting canine gastrointestinal parasites. Fecal samples from 254 dogs were processed by centrifugation-sedimentation (CS), centrifugation-flotation (CF) and a commercial assay for fecal concentration (TF-test). The following parasites were detected: Ancylostoma (37.8%), Giardia (16.9%), Toxocara canis (8.7%), Trichuris vulpis (7.1%), Isospora (3.5%), and Sarcocystis (2.7%). The calculated analytical sensitivity indicated that CF was more accurate (P<0.01) in detecting Ancylostoma, T. canis, T. vulpis and Giardia infections. However, CF showed significantly higher sensitivity only for Ancylostoma, compared to the other two methods. The kappa index value of diagnostic agreement between TF-test and CF was high for T. canis (83%) and moderate for Giardia (72%) and Ancylostoma (63%). The advantages and limitations of each method were assessed for individual diagnosis and epidemiological investigation.
本研究旨在比较商业检测试剂盒与两种常规粪便浓缩方法检测犬胃肠道寄生虫的效率。对 254 只犬的粪便样本进行离心沉淀（CS）、离心浮聚（CF）和商业粪便浓缩检测（TF-试验）。检测到的寄生虫包括：钩虫（37.8%）、贾第虫（16.9%）、犬弓首蛔虫（8.7%）、毛首线虫（7.1%）、等孢球虫（3.5%）和肉孢子虫（2.7%）。分析灵敏度的计算结果表明，CF 在检测钩虫、犬弓首蛔虫、毛首线虫和贾第虫感染方面更准确（P<0.01）。然而，与其他两种方法相比，CF 仅对钩虫的检测显示出显著更高的灵敏度。TF-试验和 CF 之间的诊断一致性kappa 指数值对犬弓首蛔虫（83%）和贾第虫（72%）和钩虫（63%）的诊断具有较高的一致性。对每种方法的个体诊断和流行病学调查的优缺点进行了评估。
